LOCUS       sequence-79059-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag
            in mammalian cells. cDNA is synthetic, containing convenient 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Monette MY, Somasekharan S, Forbush B
  TITLE     Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion 
            Transport and Transporter Activation Revealed by Internal 
            Crosslinking between Transmembrane Domains 10 and 11/12.
  JOURNAL   J Biol Chem. 2014 Jan 22.
  PUBMED    24451383
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 Jan 22."
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgtccacaga tcatcaaacc aacatttttt gtgaaatcgt gaacaagatg aagtaaggcc
       61 ggccgtgagt ttggcgctcc tgtcataaca agacactgtg gcctaaagtt tttcacatgg
      121 tcctccacgc cagaaagcct aatactatgt tgcagggcat tgaggtaagt cagggcttgt
      181 gtagaggatc cccaattcac atcgggtttt ttgtaggtaa cataaatata cagcccaagg
      241 actatcacgt aggttagcaa agcagcccac cagttaatga cgaacattac tatgcaacaa
      301 agaattgctc caagaagtga gatccacata ttgtagtatt taaatgccgg tcgccatcct
      361 gggcttttgg ccagtgaagc atggaatact gaaaaattga tgagggcata gctggcaagg
      421 aagaagtttg agataattgg tgcacaaaca ttcagttcgg caattaagat gaatccaagt
      481 gcaattaaga atgttaagat gtagccacgt aatggctcat tatttttccc ataaccttta
      541 gcaaacatct ggaaagcggg gtagatgttg tctttacata gggcctgaaa tatcttggga
      601 gcactcacta gggatgctaa tgcgctagaa agagtggcag agaatatgcc cgcggaaatt
      661 agtggtgtaa atcctgacac catactcatt acctgaaagt tgttcattag gccgtagcta
      721 caaggactgg attcacaaga tgaaaaatca aagtttaatt tgcaggctgc gctagtacag
      781 tttgttagct ctgttacgat agtgtcatta acattaccag tggcatcgcg tacgacacaa
      841 gaacctacag acacggcaat tcctacataa accaatgtag taattaaaat ggctaggagt
      901 gttcctttgg gtatggctga ctga
//
