LOCUS       sequence-79073-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a catalytically inactive, human codon-optimized Cas9 
            under the control of Murine Stem Cell retroVirus LTR promoter for 
            mammalian gene knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim 
            WA
  TITLE     Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific 
            Control of Gene Expression.
  JOURNAL   Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
  PUBMED    23452860
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.02"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1173-83"
COMMENT     Sequence Label: pdCas9-humanized
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(59..79)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(83..103)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(107..127)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta gaattctata
       61 cctttctctt cttttttgga tctacctttc tcttcttttt tggatctacc tttctcttct
      121 tttttggatc agctccctca tcccctccga gctgtgagag gtcaatccga gtttcgtaaa
      181 gtccggtgat gctctggtgg atgagggtgg cgtccagcac ttctttggtg gaggtatagc
      241 gtttccggtc gatggtggtg tcgaagtact tgaatgcggc aggggcacca agattggtga
      301 gggtgaacag gtggatgata ttctcagcct gctcccgaat tggtttgtct ctgtgcttat
      361 tgtatgcgct aaggacttta tcgaggttgg catcagcgag aatgacgcgt ttagaaaact
      421 cggaaatctg ctcaatgatc tcgtccagat aatgcttatg ctgctccaca aacagttgct
      481 tttgctcgtt gtcctcgggg ctccccttca gcttctcgta atggcttgca aggtagagga
      541 aattcacgta cttggagggg agagccagct cattaccctt ctgaagttcg ccagcggagg
      601 cgagcatccg cttccgccca ttctccagtt cgaacaggct atactttggg agcttgatga
      661 taaggtcctt cttgacttcc ttgtaacctt tggcttcgag aaagtcaatt gggttcttct
      721 cgaaggagct gcgctccata atggtgatac ccagcagttc cttcacgctc ttgagcttct
      781 tagactttcc tttttccacc ttagccacga ccaggacaga ataagccaca gttggtgaat
      841 cgaatccgcc gtacttctta gggtcccaat ccttcttcct tgcaatgagc ttgtcgga
//
