LOCUS       sequence-79092-e        4809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast marker swap plasmid for converting LEU2 to KanMX3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Voth WP, Jiang YW, Stillman DJ
  TITLE     New 'marker swap' plasmids for converting selectable markers on 
            budding yeast gene disruptions and plasmids.
  JOURNAL   Yeast. 2003 Aug;20(11):985-93.
  PUBMED    12898713
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2003-08"; volume: "20(11)"; pages: "985-93"
COMMENT     Sequence Label: M3926 leu2::KanMX3 Disruptor Converter
FEATURES             Location/Qualifiers
     source          1..4809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..442
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(2193..2209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2193..2209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2206..2228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2217..2233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2241..2247,2248..2265,2266..2271))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2286..2307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2424..2441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2595..3183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2675..2694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3354..4145,4146..4214))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3977..3996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4215..4319)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4387..4405
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4443..4465)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4565..4584
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4778..4800
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4792..4809
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4793..4809
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccgg ggatccggtg attgattgag caagctaact gtgggaatac tcaggtatcg
       61 taagatgcaa gagttcgaat ctcttagcaa ccattatttt tttcctcaac ataacgagaa
      121 cacacagggg cgctatcgca cagaatcaaa ttcgatgact ggaaattttt tgttaatttc
      181 agaggtcgcc tgacgcatat acctttttca actgaaaaat tgggagaaaa aggaaaggtg
      241 agagcgccgg aaccggcttt tcatatagaa tagagaagcg ttcatgacta aatgcttgca
      301 tcacaatact tgaagttgac aatattattt aaggacctat tgttttttcc aataggtggt
      361 tagcaatcgt cttactttct aacttttctt accttttaca tttcagcaat atatatatat
      421 atttcaagga tataccattc taatgtctgc ccctaagaag atcgtcgttt tgccaggtga
      481 ccacgttggt caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt
      541 tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc
      601 tacaggtgtt ccacttccag atgaggcgct ggaagcctcc aagaaggctg atgccgtttt
      661 gttaggtgct gtgggtggtc ctaaatgggg taccggtagt gttagacctg aacaaggttt
      721 actaaaaatc cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc
      781 cgactctctt ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt
      841 tgttgtcaga gaattagtgg gaggtattta ctttggtaag agaaaggaag acgatggtga
      901 tggtgtcgct tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat
      961 ggccgctttc atggccctac aacatgagcc accattgcct atttggtcct tggataaagc
     1021 taatgttttg gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga
     1081 attccctaca ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa
     1141 gaacccaacc cacctaaatg gtattataat caccagcaac atgtttggtg atgatctgcc
     1201 cgccgggaag gcgaacccga tcggatgcat cctctctgct gccatgatgc tgaagttgtc
     1261 gttgaacatg gttgctgccg gcgaggcggt cgagcaggca gtgcaggagg tgttggactc
     1321 gggagtcaga acgggcgacc tgctcggctc gagctccact tcggaggttg gcgacgccat
     1381 tgcgcttgca gttaaggaag ccttgcgcag gcaatccgca gctggtctga gctagcctcg
     1441 aggacccttc tctttagact attctactct tatgcacgta aaaaattcta ggaaatatgt
     1501 attaactaga agtaaaataa ccggctagtg gcattcatat agccgtctgt ttacatctac
     1561 atcacacatt tcgagtgtat atctcgcaac gttggcgtta aataggcagt caatggcccg
     1621 accattctat ggtgtttagg tcgatgccat ctttgtacgt ttaaacggta aacgagctcg
     1681 aattatcatc tccgatgaag cctccgttat cccaggttcc ttgggtttgt tgccatctgc
     1741 gtccttggcc tctttgccag acaagaacac cgcatttggt ttgtacgaac catgccacgg
     1801 ttctgctcca gatttgccaa agaataaggt caaccctatc gccactatct tgtctgctgc
     1861 aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt aaggccattg aagatgcagt
     1921 taaaaaggtt ttggatgcag gtatcagaac tggtgattta ggtggttcca acagtaccac
     1981 cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa aagattctct
     2041 ttttttatga tatttgtaca taaactttat aaatgaaatt cataatagaa acgacacgaa
     2101 attacaaaat ggaatatgtt catagggtag agcttgctca atcaatcacc ggatccgtcg
     2161 acctgcagcc aagctagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     2221 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     2281 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2341 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2401 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3001 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     3061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3241 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3301 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3361 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3421 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3481 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3541 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3601 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3661 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3721 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3781 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3841 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3901 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3961 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4021 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4081 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4141 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4201 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4261 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4321 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     4381 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     4441 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     4501 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     4561 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     4621 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     4681 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     4741 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     4801 acggccagt
//
