LOCUS       sequence-79093-e       10119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast marker swap plasmid for converting LEU2 to LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Voth WP, Jiang YW, Stillman DJ
  TITLE     New 'marker swap' plasmids for converting selectable markers on 
            budding yeast gene disruptions and plasmids.
  JOURNAL   Yeast. 2003 Aug;20(11):985-93.
  PUBMED    12898713
REFERENCE   2  (bases 1 to 10119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2003-08"; volume: "20(11)"; pages: "985-93"
COMMENT     Sequence Label: D771 leu2::LYS2 Disruptor Converter
FEATURES             Location/Qualifiers
     source          1..10119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1746..1985
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1986..6164
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(7898..7914)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7898..7914)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7911..7933)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7922..7938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7946..7952,7953..7970,7971..7976))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7991..8012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8129..8146)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8300..8888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8380..8399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9059..9850,9851..9919))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9682..9701
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9920..10024)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10092..10110
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttcgcga gctcgaggag
      421 aacttctagt atatccacat acctaatatt attgccttat taaaaatgga atcccaacaa
      481 ttacatcaaa atccacattc tcttcaaaat caattgtcct gtacttcctt gttcatgtgt
      541 gttcaaaaac gttatattta taggataatt atactctatt tctcaacaag taattggttg
      601 tttggccgag cggtctaagg cgcctgattc aagaaatatc ttgaccgcag ttaactgtgg
      661 gaatactcag gtatcgtaag atgcaagagt tcgaatctct tagcaaccat tatttttttt
      721 ctctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg
      781 aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg
      841 ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata gagaagcgtt
      901 catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg
      961 ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt
     1021 tcagcaatat atatatatat atatatatat atttcaagga tataccattg taatgtctgc
     1081 ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc
     1141 cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa
     1201 tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct
     1261 ggaagcctcc aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg
     1321 taccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta
     1381 cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa
     1441 gccacaattt gctaaaggta ctgacttcgt tgttgttaga gaattagtgg gaggtattta
     1501 ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac
     1561 cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc
     1621 accattgcct atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag
     1681 aaaaactgtg gaggaaacca tcaagaacga attctcgacg gatccggtga ttgattgagc
     1741 aagctaattc cacttgcaat tacataaaaa attccggcgg tttttcgcgt gtgactcaat
     1801 gtcgaaatac ctgcctaatg aacatgaaca tcgcccaaat gtatttgaag acccgctggg
     1861 agaagttcaa gatatataag taacaagcag ccaatagtat aaaaaaaaat ctgagtttat
     1921 tacctttcct ggaatttcag tgaaaaactg ctaattatag agagatatca cagagttact
     1981 cactaatgac taacgaaaag gtctggatag agaagttgga taatccaact ctttcagtgt
     2041 taccacatga ctttttacgc ccacaacaag aaccttatac gaaacaagct acatattcgt
     2101 tacagctacc tcagctcgat gtgcctcatg atagtttttc taacaaatac gctgtcgctt
     2161 tgagtgtatg ggctgcattg atatatagag taaccggtga cgatgatatt gttctttata
     2221 ttgcgaataa caaaatctta agattcaata ttcaaccaac gtggtcattt aatgagctgt
     2281 attctacaat taacaatgag ttgaacaagc tcaattctat tgaggccaat ttttcctttg
     2341 acgagctagc tgaaaaaatt caaagttgcc aagatctgga aaggacccct cagttgttcc
     2401 gtttggcctt tttggaaaac caagatttca aattagacga gttcaagcat catttagtgg
     2461 actttgcttt gaatttggat accagtaata atgcgcatgt tttgaactta atttataaca
     2521 gcttactgta ttcgaatgaa agagtaacca ttgttgcgga ccaatttact caatatttga
     2581 ctgctgcgct aagcgatcca tccaattgca taactaaaat ctctctgatc accgcatcat
     2641 ccaaggatag tttacctgat ccaactaaga acttgggctg gtgcgatttc gtggggtgta
     2701 ttcacgacat tttccaggac aatgctgaag ccttcccaga gagaacctgt gttgtggaga
     2761 ctccaacact aaattccgac aagtcccgtt ctttcactta tcgcgacatc aaccgcactt
     2821 ctaacatagt tgcccattat ttgattaaaa caggtatcaa aagaggtgat gtagtgatga
     2881 tctattcttc taggggtgtg gatttgatgg tatgtgtgat gggtgtcttg aaagccggcg
     2941 caaccttttc agttatcgac cctgcatatc ccccagccag acaaaccatt tacttaggtg
     3001 ttgctaaacc acgtgggttg attgttatta gagctgctgg acaattggat caactagtag
     3061 aagattacat caatgatgaa ttggagattg tttcaagaat caattccatc gctattcaag
     3121 aaaatggtac cattgaaggt ggcaaattgg acaatggcga ggatgttttg gctccatatg
     3181 atcactacaa agacaccaga acaggtgttg tagttggacc agattccaac ccaaccctat
     3241 ctttcacatc tggttccgaa ggtattccta agggtgttct tggtagacat ttttccttgg
     3301 cttattattt caattggatg tccaaaaggt tcaacttaac agaaaatgat aaattcacaa
     3361 tgctgagcgg tattgcacat gatccaattc aaagagatat gtttacacca ttatttttag
     3421 gtgcccaatt gtatgtccct actcaagatg atattggtac accgggccgt ttagcggaat
     3481 ggatgagtaa gtatggttgc acagttaccc atttaacacc tgccatgggt caattactta
     3541 ctgcccaagc tactacacca ttccctaagt tacatcatgc gttctttgtg ggtgacattt
     3601 taacaaaacg tgattgtctg aggttacaaa ccttggcaga aaattgccgt attgttaata
     3661 tgtacggtac cactgaaaca cagcgtgcag tttcttattt cgaagttaaa tcaaaaaatg
     3721 acgatccaaa ctttttgaaa aaattgaaag atgtcatgcc tgctggtaaa ggtatgttga
     3781 acgttcagct actagttgtt aacaggaacg atcgtactca aatatgtggt attggcgaaa
     3841 taggtgagat ttatgttcgt gcaggtggtt tggccgaagg ttatagagga ttaccagaat
     3901 tgaataaaga aaaatttgtg aacaactggt ttgttgaaaa agatcactgg aattatttgg
     3961 ataaggataa tggtgaacct tggagacaat tctggttagg tccaagagat agattgtaca
     4021 gaacgggtga tttaggtcgt tatctaccaa acggtgactg tgaatgttgc ggtagggctg
     4081 atgatcaagt taaaattcgt gggttcagaa tcgaattagg agaaatagat acgcacattt
     4141 cccaacatcc attggtaaga gaaaacatta ctttagttcg caaaaatgcc gacaatgagc
     4201 caacattgat cacatttatg gtcccaagat ttgacaagcc agatgacttg tctaagttcc
     4261 aaagtgatgt tccaaaggag gttgaaactg accctatagt taagggctta atcggttacc
     4321 atcttttatc caaggacatc aggactttct taaagaaaag attggctagc tatgctatgc
     4381 cttccttgat tgtggttatg gataaactac cattgaatcc aaatggtaaa gttgataagc
     4441 ctaaacttca attcccaact cccaagcaat taaatttggt agctgaaaat acagtttctg
     4501 aaactgacga ctctcagttt accaatgttg agcgcgaggt tagagactta tggttaagta
     4561 tattacctac caagccagca tctgtatcac cagatgattc gtttttcgat ttaggtggtc
     4621 attctatctt ggctaccaaa atgattttta ccttaaagaa aaagctgcaa gttgatttac
     4681 cattgggcac aattttcaag tatccaacga taaaggcctt tgccgcggaa attgacagaa
     4741 ttaaatcatc gggtggatca tctcaaggtg aggtcgtcga aaatgtcact gcaaattatg
     4801 cggaagacgc caagaaattg gttgagacgc taccaagttc gtacccctct cgagaatatt
     4861 ttgttgaacc taatagtgcc gaaggaaaaa caacaattaa tgtgtttgtt accggtgtca
     4921 caggatttct gggctcctac atccttgcag atttgttagg acgttctcca aagaactaca
     4981 gtttcaaagt gtttgcccac gtcagggcca aggatgaaga agctgcattt gcaagattac
     5041 aaaaggcagg tatcacctat ggtacttgga acgaaaaatt tgcctcaaat attaaagttg
     5101 tattaggcga tttatctaaa agccaatttg gtctttcaga tgagaagtgg atggatttgg
     5161 caaacacagt tgatataatt atccataatg gtgcgttagt tcactgggtt tatccatatg
     5221 ccaaattgag ggatccaaat gttatttcaa ctatcaatgt tatgagctta gccgccgtcg
     5281 gcaagccaaa gttctttgac tttgtttcct ccacttctac tcttgacact gaatactact
     5341 ttaatttgtc agataaactt gttagcgaag ggaagccagg cattttagaa tcagacgatt
     5401 taatgaactc tgcaagcggg ctcactggtg gatatggtca gtccaaatgg gctgctgagt
     5461 acatcattag acgtgcaggt gaaaggggcc tacgtgggtg tattgtcaga ccaggttacg
     5521 taacaggtgc ctctgccaat ggttcttcaa acacagatga tttcttattg agatttttga
     5581 aaggttcagt ccaattaggt aagattccag atatcgaaaa ttccgtgaat atggttccag
     5641 tagatcatgt tgctcgtgtt gttgttgcta cgtctttgaa tcctcccaaa gaaaatgaat
     5701 tggccgttgc tcaagtaacg ggtcacccaa gaatattatt caaagactac ttgtatactt
     5761 tacacgatta tggttacgat gtcgaaatcg aaagctattc taaatggaag aaatcattgg
     5821 aggcgtctgt tattgacagg aatgaagaaa atgcgttgta tcctttgcta cacatggtct
     5881 tagacaactt acctgaaagt accaaagctc cggaactaga cgataggaac gccgtggcat
     5941 ctttaaagaa agacaccgca tggacaggtg ttgattggtc taatggaata ggtgttactc
     6001 cagaagaggt tggtatatat attgcatttt taaacaaggt tggattttta cctccaccaa
     6061 ctcataatga caaacttcca ctgccaagta tagaactaac tcaagcgcaa ataagtctag
     6121 ttgcttcagg tgctggtgct cgtggaagct ccgcagcagc ttaaggttga gcattacgta
     6181 tgatatgtcc atgtacaata attaaatatg aattaggaga aagacttagc ttcttttcgg
     6241 gtgatgtcac ttaaaaactc cgagaataat atataataag agaataaaat attagttatt
     6301 gaataagaac tgtaaatcag ctggcgttag tctgctaatg gcagcttcat cttggtttat
     6361 tgtagcatga atcatatttg cctttttttc ctgtaattca atgattcttg cttctatact
     6421 atcctcaatg caaaaccttg tgatcttcac aggtcgatac tgaccaattc tatgaactct
     6481 atcaccactt tgccattcaa cactagggtt ccaccatggg tctaaaatga atacttgcga
     6541 agcttcacaa agattcaaag caacaccgcc cgcctttaaa ctgaccaaga aaacctcgca
     6601 ttgaatgttg ttcatgaaat acttgatggt ttcatctctt tgcgtcggtg acatactacc
     6661 ctgaagcttc actgtttgaa atccagctct tttcaatctc cactctacca gatccagcat
     6721 actggtaaac tgggaaaaca caatggattt aatcgttctc ttgttgcttc tcagtttgta
     6781 tagttcttcc acaagtgctt cgattttcgt tgatgattgc cacttgccac tcatgtttag
     6841 acggctaaca atactttgct ttttgaagga atcaaggtcc acttccaaag caggttgaga
     6901 caaatcgagc ttgctcaatc aatcaccgga tccccgggaa ttccctacat tgaaggttca
     6961 acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc acctaaatgg
     7021 tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct ccgttatccc
     7081 aggctccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca agaacaccgc
     7141 atttggtttg tacgaaccat gccatggttc cgctccagat ttgccaaaga ataaggtcaa
     7201 ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga acttgcctga
     7261 agaaggtaaa gccattgaag atgcagttaa aaaggttttg gatgcaggta tcagaactgg
     7321 tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg aagaagttaa
     7381 gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa
     7441 tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg
     7501 aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga
     7561 atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt
     7621 aacattaata ttgacaagga ggagggcatc acacaaaaag ttaggtgtaa cagaaaatca
     7681 tgaaactatg atttctaatt tatatattgg aggattttct ctaaaaaaaa aaatacaaca
     7741 aataaaaaac actcaatgac ctgaccattt gatggagttt aagtcaatac cttcttgaac
     7801 catttcccat aatggtgaaa gttccctcaa gaattttact ctgtcagaaa cggccttacg
     7861 acgtagtcga cctgcagcca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7921 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7981 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8041 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8101 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8161 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8221 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8281 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8341 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8401 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8461 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     8521 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8581 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8641 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8701 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     8761 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8821 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8881 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8941 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9001 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9061 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9121 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9181 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9241 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9301 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9361 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9421 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9481 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9541 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9601 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9661 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9721 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9781 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9841 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9901 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9961 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10021 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    10081 taacctataa aaataggcgt atcacgaggc cctttcgtc
//
