LOCUS       sequence-79094-e        7268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast marker swap plasmid for converting TRP1 to ADE2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Voth WP, Jiang YW, Stillman DJ
  TITLE     New 'marker swap' plasmids for converting selectable markers on 
            budding yeast gene disruptions and plasmids.
  JOURNAL   Yeast. 2003 Aug;20(11):985-93.
  PUBMED    12898713
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2003-08"; volume: "20(11)"; pages: "985-93"
COMMENT     Sequence Label: M3938 trp1::ADE2 Disruptor Converter
FEATURES             Location/Qualifiers
     source          1..7268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..138
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     promoter        1337..1712
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     CDS             1713..3428
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     CDS             complement(3727..3738)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4652..4668)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4652..4668)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4665..4687)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4676..4692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4700..4706,4707..4724,4725..4730))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4745..4766
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4883..4900)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5054..5642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5134..5153)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5813..6604,6605..6673))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6436..6455
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6674..6778)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6846..6864
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6902..6924)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7024..7043
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7237..7259
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7251..7268
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7252..7268
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccgg ggatccggtg attgattgag caagctaatt cggtcgaaaa aagaaaagga
       61 gagggccaag agggagggca ttggtgacta ttgagcacgt gagtatacgt gattaagcac
      121 acaaaggcag cttggagtat gtctgttatt aatttcacag gtagttctgg tccattggtg
      181 aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg ctctagattc cgatgctgac
      241 ttgctgggta ttatatgtgt gcccaataga aagagaacaa ttgacccggt tattgcaagg
      301 aaaatttcaa gtcttgtaaa agcatataaa aatagttcag gcactccgaa atacttggtt
      361 ggcgtgtttc gtaatcaacc taaggaggat gttttggctc tggtcaatga ttacggcatt
      421 gatgatccgg gatcttttta ctcaaattta cagttttgat atcttggcgt ttttcctcac
      481 atttattaca aaagaaagtg ggcaggattt gcccttcaca aaatctctgt ccaggagaag
      541 aacgtatttg accagaattg ctcaatcttc ttcattgaga cagtttttac aactgagtac
      601 acgccctaat ataaggattc tgggaggtaa tgggcagggt actggaaatt ccaattatcc
      661 cgaatttaca aacgcatcct cagtcatctc acccaagcta agggcttctc ctctactaga
      721 aagaagggcg tcaaaaatct gctatgctat tacgaaatta ctctataggt tagaatgtca
      781 tcccataggt atggcccttt taaagaagta tctccacaat caattgcgag aagcctatct
      841 agaatcaaaa cgacacttta tttccaaaaa gggagattcg accaacactt cctctaccat
      901 tgcatcatca tctttcgctg gcgcatctgt tcctctatct tcaaacgaat caggaatgct
      961 aaacggcttg aagcaaatta acgaacaaca agaatctaca ttagaaacca ctcaaaagga
     1021 agactagtaa cgccgtatcg tgattaacgt attacataag ttacaggatt catgcttatg
     1081 ggttagctat ttcgcccaat gtgtccatct gacattacta ttttgcattt taatttaatt
     1141 agaacttgac tagcgcacta ccagtatatc atctcatttc cgtaaatacc aaatgtatta
     1201 tatattgaaa gcttttgacc aggttattat aaaagaaact tcatgctcga aaaagatcat
     1261 ttcgaaaagt tgcctagttt catgaaattt taaagcagtt tatataaatt ttaccttttg
     1321 atgcggaatt gactttttct tgaataatac ataacttttc ttaaaagaat caaagacaga
     1381 taaaatttaa gagatattaa atattagtga gaagccgaga attttgtaac accaacataa
     1441 cactgacatc tttaacaact tttaattatg atacatttct tacgtcatga ttgattatta
     1501 cagctatgct gacaaatgac tcttgttgca tggctacgaa ccgggtaata ctaagtgatt
     1561 gactcttgct gaccttttat taagaactaa atggacaata ttatggagca tttcatgtat
     1621 aaattggtgc gtaaaatcgt tggatctctc ttctaagtac atcctactat aacaatcaag
     1681 aaaaacaaga aaatcggaca aaacaatcaa gtatggattc tagaacagtt ggtatattag
     1741 gagggggaca attgggacgt atgattgttg aggcagcaaa caggctcaac attaagacgg
     1801 taatactaga tgctgaaaat tctcctgcca aacaaataag caactccaat gaccacgtta
     1861 atggctcctt ttccaatcct cttgatatcg aaaaactagc tgaaaaatgt gatgtgctaa
     1921 cgattgagat tgagcatgtt gatgttccta cactaaagaa tcttcaagta aaacatccca
     1981 aattaaaaat ttacccttct ccagaaacaa tcagattgat acaagacaaa tatattcaaa
     2041 aagagcattt aatcaaaaat ggtatagcag ttacccaaag tgttcctgtg gaacaagcca
     2101 gtgagacgtc cctattgaat gttggaagag atttgggttt tccattcgtc ttgaagtcga
     2161 ggactttggc atacgatgga agaggtaact tcgttgtaaa gaataaggaa atgattccgg
     2221 aagctttgga agtactgaag gatcgtcctt tgtacgccga aaaatgggca ccatttacta
     2281 aagaattagc agtcatgatt gtgagatctg ttaacggttt agtgttttct tacccaattg
     2341 tagagactat ccacaaggac aatatttgtg acttatgtta tgcgcctgct agagttccgg
     2401 actccgttca acttaaggcg aagttgttgg cagaaaatgc aatcaaatct tttcccggtt
     2461 gtggtatatt tggtgtggaa atgttctatt tagaaacagg ggaattgctt attaacgaaa
     2521 ttgccccaag gcctcacaac tctggacatt ataccattga tgcttgcgtc acttctcaat
     2581 ttgaagctca tttgagatca atattggatt tgccaatgcc aaagaatttc acatctttct
     2641 ccaccattac aacgaacgcc attatgctaa atgttcttgg agacaaacat acaaaagata
     2701 aagagctaga aacttgcgaa agagcattgg cgactccagg ttcctcagtg tacttatatg
     2761 gaaaagagtc tagacctaac agaaaagtag gtcacataaa tattattgcc tccagtatgg
     2821 cggaatgtga acaaaggctg aactacatta caggtagaac tgatattcca atcaaaatct
     2881 ctgtcgctca aaagttggac ttggaagcaa tggtcaaacc attggttgga atcatcatgg
     2941 gatcagactc tgacttgccg gtaatgtctg ccgcatgtgc ggttttaaaa gattttggcg
     3001 ttccatttga agtgacaata gtctctgctc atagaactcc acataggatg tcagcatatg
     3061 ctatttccgc aagcaagcgt ggaattaaaa caattatcgc tggagctggt ggggctgctc
     3121 acttgccagg tatggtggct gcaatgacac cacttcctgt catcggtgtg cccgtaaaag
     3181 gttcttgtct agatggagta gattctttac attcaattgt gcaaatgcct agaggtgttc
     3241 cagtagctac cgtcgctatt aataatagta cgaacgctgc gctgttggct gtcagactgc
     3301 ttggcgctta tgattcaagt tatacaacga aaatggaaca gtttttatta aagcaagaag
     3361 aagaagttct tgtcaaagca caaaagttag aaactgtcgg ttacgaagct tatctagaaa
     3421 acaagtaata tataagttta ttgatatact tgtacagcaa ataattataa aatgatatac
     3481 ctatttttta ggctttgtta tgattacatc aaatgtggac ttcatacata gaaatcaacg
     3541 cttacaggtg tcctttttta agaatttcat acataagatc acttattata catacataca
     3601 tatccagtaa caagaagcaa ggaataatta cctgcttaag tctgcgatta aaaaaataac
     3661 gtttcgatac agttcatata aggcggctca atgcagaacc gaggatagcg ctacgtcagg
     3721 atatctttgt agttcccaaa tataaatgcg acaatatagt ttctttcttt catatcaata
     3781 atatcctttt ctccactgaa atcacgaatc aaacctggag caaaaactaa agccaaatta
     3841 taaagcgtca ttcgattcca gtgactgtac cgtgtaacct tttctatatg ttcactcagt
     3901 actcttaaca ccctataatg ttcccttgga aggtcttcca atatgttttt taaagcgctc
     3961 ttgctcgaca tataagtgtc cgaattcttt gcttctaagg acaactttcc tccaacaaat
     4021 ggcaagtttt ccatcatttt tttagattta actaacctca tcaacggctc gtatatttga
     4081 aaggtaaaga tagggttggg gagctttctt aagtatcgct tcaacacacc agtaacaacg
     4141 ttgagatccc ggatcatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag
     4201 ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc
     4261 agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg
     4321 acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc
     4381 gaaagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt
     4441 agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa
     4501 gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat tactgagtag
     4561 tatttattta agtattgttt gtgcacttgc ccttgctcaa tcaatcaccg gatccgtcga
     4621 cctgcagcca agctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     4681 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4741 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     4801 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4861 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4921 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4981 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5041 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5101 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5161 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5221 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5281 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5341 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5401 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5461 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     5521 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5581 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5641 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5701 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5761 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5821 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5881 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5941 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6001 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6061 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6121 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6181 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6241 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6301 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6361 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6421 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6481 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6541 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6601 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6661 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6721 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6781 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     6841 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     6901 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6961 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     7021 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7081 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     7141 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     7201 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     7261 cggccagt
//
