LOCUS       sequence-79096-e        7034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Voth WP, Jiang YW, Stillman DJ
  TITLE     New 'marker swap' plasmids for converting selectable markers on 
            budding yeast gene disruptions and plasmids.
  JOURNAL   Yeast. 2003 Aug;20(11):985-93.
  PUBMED    12898713
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2003-08"; volume: "20(11)"; pages: "985-93"
COMMENT     Sequence Label: M4786 KanMX::hisG-URA3-HisG Disruptor Converter
FEATURES             Location/Qualifiers
     source          1..7034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(569..789)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     terminator      2048..2245
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2352..2371)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2353..2371)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2458..2475)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2629..3217)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2709..2728)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3388..4179,4180..4248))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4011..4030
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4249..4353)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4421..4439
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4477..4499)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4599..4618
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4699..4717
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4699..4716
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        4795..5138
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             complement(join(6799..7034,1..568))
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt
       61 caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta
      121 acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa
      181 caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt
      241 atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt
      301 cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca
      361 tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg
      421 cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg
      481 tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag
      541 cacgttcctt atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc
      601 tgtgcagttg ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta
      661 tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg
      721 aatcaaaaaa atttcaaaga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat
      781 tgaattgaaa agcttatcga tgataagctg tcaaacatga gaattaattc agatccgctg
      841 ttccagtcaa tcagggtatt gaagctcatg gtctttactc catcacaggg ttccgcctta
      901 tccggcctac agaacccaaa atatcaacgc attacgtagg cctgataagc gcagcgccat
      961 caggcgtcag atcactccat catcttctcg atcggcagta ccagaatcga gctggcgcca
     1021 agcgctttca gtttctccat ggtttcccag aacaacgttt cgctgctgac catgtgcatc
     1081 gccacgcgct gttgctcgcc tgccagcggc agaattgtcg gcctttcggc gcctggcagc
     1141 agggcgataa cctcttccag gcgttcactt ggcgcgtgca tcatgatgta tttcgattcg
     1201 cgcgcctgaa tcacgccctg aatacgggtc agcaatttat cgatcagctc ttgcttgctc
     1261 tgtgccatct caccgtcgcg ctgaatcaga caggctttag agcggtagat aacttcgact
     1321 tcacgcaggc cgttagcttc aagcgtcgcg ccggtagaga ccaaatcgca gatagcgtcg
     1381 gccagccccg cgcgcggcgc gacttcgaca gaaccattta acagacacga tttaaaagag
     1441 acgcctttct ggtcgaggta gcgtttgagg aggtgcggat atgaggtagc gatacgttta
     1501 ccgtccagcg cggccgggcc gtcccaggct tcgtcaaccg gtgttgccag cgataaacgg
     1561 cagccgccga agtcaagacg gcgcagggtt aaatagcgtg gatcttcgcc ctgtgcgcgg
     1621 cggttgagta gctcttcttc cagcacgttt tcgccgataa taccgagatc gaccacgcca
     1681 tccattacca gacccggaat gtcatcatca cgcacgcgca ggatatcaat cggcatgttt
     1741 tccgccatcg caatcaggcg ctgagtgtgt aaattaattt ttatgccgca gcgggccagc
     1801 aattctcgtg aatcatcgct taaacggcct gatttctgaa tagctatgcg taagcgggtg
     1861 ttgtctaaca ttctgcgttc ctctttatcc tgtctgaacc ggtctgtatc gcgcgccaaa
     1921 aaaaaagccc ccggaagatg atcttccggg ggctttctca tgcgttcatg caccactgga
     1981 agatctgaat tcgatatcaa gcttatcgat accgtcgacc tgcaggcatg ccctgcccct
     2041 aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt
     2101 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct
     2161 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg
     2221 tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagtgtc
     2281 gaaaacgagc tcgaattcat cgatgatatc agatccacta gtggcctatg cggccgcgga
     2341 tctgccggtc tccctatagt gagtcgtatt aatttcgata agccaggtta acctgcatta
     2401 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2461 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2521 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2581 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2641 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2701 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2761 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2821 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2881 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2941 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3001 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3061 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3121 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3181 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3241 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3301 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3361 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3421 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3481 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3541 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3601 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3661 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3721 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3781 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3841 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3901 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3961 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4021 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4081 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4141 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4201 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4261 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4321 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4381 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     4441 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4501 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4561 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     4621 gagagtgcac catatggaca tattgtcgtt agaacgcggc tacaattaat acataacctt
     4681 atgtatcata cacatacgat ttaggtgaca ctatagaacg cggccgccag ctgaagctta
     4741 aggcgcgcca gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
     4801 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
     4861 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
     4921 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
     4981 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
     5041 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
     5101 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtacccgg ggatccggcc
     5161 agatccggct gttccagtca atcagggtat tgaagctcat ggtctttact ccatcacagg
     5221 gttccgcctt atccggccta cagaacccaa aatatcaacg cattacgtag gcctgataag
     5281 cgcagcgcca tcaggcgtca gatcactcca tcatcttctc gatcggcagt accagaatcg
     5341 agctggcgcc aagcgctttc agtttctcca tggtttccca gaacaacgtt tcgctgctga
     5401 ccatgtgcat cgccacgcgc tgttgctcgc ctgccagcgg cagaattgtc ggcctttcgg
     5461 cgcctggcag cagggcgata acctcttcca ggcgttcact tggcgcgtgc atcatgatgt
     5521 atttcgattc gcgcgcctga atcacgccct gaatacgggt cagcaattta tcgatcagct
     5581 cttgcttgct ctgtgccatc tcaccgtcgc gctgaatcag acaggcttta gagcggtaga
     5641 taacttcgac ttcacgcagg ccgttagctt caagcgtcgc gccggtagag accaaatcgc
     5701 agatagcgtc ggccagcccc gcgcgcggcg cgacttcgac agaaccattt aacagacacg
     5761 atttaaaaga gacgcctttc tggtcgaggt agcgtttgag gaggtgcgga tatgaggtag
     5821 cgatacgttt accgtccagc gcggccgggc cgtcccaggc ttcgtcaacc ggtgttgcca
     5881 gcgataaacg gcagccgccg aagtcaagac ggcgcagggt taaatagcgt ggatcttcgc
     5941 cctgtgcgcg gcggttgagt agctcttctt ccagcacgtt ttcgccgata ataccgagat
     6001 cgaccacgcc atccattacc agacccggaa tgtcatcatc acgcacgcgc aggatatcaa
     6061 tcggcatgtt ttccgccatc gcaatcaggc gctgagtgtg taaattaatt tttatgccgc
     6121 agcgggccag caattctcgt gaatcatcgc ttaaacggcc tgatttctga atagctatgc
     6181 gtaagcgggt gttgtctaac attctgcgtt cctctttatc ctgtctgaac cggtctgtat
     6241 cgcgcgccaa aaaaaaagcc cccggaagat gatcttccgg gggctttctc atgcgttcat
     6301 gcaccactgg aagatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc
     6361 tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag
     6421 aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact
     6481 ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa
     6541 gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga
     6601 tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag
     6661 ctttttcttt ccaatttttt ttttttcgtc attataaaaa tcattacgac cgagattccc
     6721 gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt
     6781 tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc
     6841 tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct
     6901 tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg
     6961 acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta
     7021 accttcatct cttc
//
