LOCUS       sequence-79098-e        4079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast marker swap plasmid for converting KanMX3 to TRP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Voth WP, Jiang YW, Stillman DJ
  TITLE     New 'marker swap' plasmids for converting selectable markers on 
            budding yeast gene disruptions and plasmids.
  JOURNAL   Yeast. 2003 Aug;20(11):985-93.
  PUBMED    12898713
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2003-08"; volume: "20(11)"; pages: "985-93"
COMMENT     Sequence Label: M4757 KanMX::TRP1 Disruptor Converter
FEATURES             Location/Qualifiers
     source          1..4079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..423
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             complement(596..1270)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(1271..1372)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     terminator      1412..1609
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1716..1735)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1717..1735)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1822..1839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1993..2581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2073..2092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2752..3543,3544..3612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3375..3394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3613..3717)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3785..3803
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3841..3863)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3963..3982
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(4063..4079,1..2)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(4063..4079,1)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttaaggcg cgccagatct gtttagcttg cctcgtcccc
       61 gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt
      121 gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt
      181 ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc
      241 ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca
      301 cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca
      361 tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca
      421 accatgggta cccggggatc ctctagacaa tcaatcaaaa agccaaatga tttagcatta
      481 tctttacatc ttgttatttt acagatttta tgtttagatc ttttatgctt gcttttcaaa
      541 aggcctgcag gcaagtgcac aaacaatact taaataaata ctactcagta ataacctatt
      601 tcttagcatt tttgacgaaa tttgctattt tgttagagtc ttttacacca tttgtctcca
      661 cacctccgct tacatcaaca ccaataacgc catttaatct aagcgcatca ccaacatttt
      721 ctggcgtcag tccaccagct aacataaaat gtaagctttc ggggctctct tgccttccaa
      781 cccagtcaga aatcgagttc caatccaaaa gttcacctgt cccacctgct tctgaatcaa
      841 acaagggaat aaacgaatga ggtttctgtg aagctgcact gagtagtatg ttgcagtctt
      901 ttggaaatac gagtctttta ataactggca aaccgaggaa ctcttggtat tcttgccacg
      961 actcatctcc atgcagttgg acgatatcaa tgccgtaatc attgaccaga gccaaaacat
     1021 cctccttagg ttgattacga aacacgccaa ccaagtattt cggagtgcct gaactatttt
     1081 tatatgcttt tacaagactt gaaattttcc ttgcaataac cgggtcaatt gttctctttc
     1141 tattgggcac acatataata cccagcaagt cagcatcgga atctagagca cattctgcgg
     1201 cctctgtgct ctgcaagccg caaactttca ccaatggacc agaactacct gtgaaattaa
     1261 taacagacat actccaagct gcctttgtgt gcttaatcac gtatactcac gtgctcaata
     1321 gtcaccaatg ccctccctct tggccctctc cttttctttt ttcgaccgaa ttctagagtc
     1381 gacctgcagg catgccctgc ccctaatcag tactgacaat aaaaagattc ttgttttcaa
     1441 gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag
     1501 cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca
     1561 gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg
     1621 atactaacgc cgccatccag tgtcgaaaac gagctcgaat tcatcgatga tatcagatcc
     1681 actagtggcc tatgcggccg cggatctgcc ggtctcccta tagtgagtcg tattaatttc
     1741 gataagccag gttaacctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1801 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2161 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2401 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2641 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2701 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2761 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2821 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2881 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2941 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3001 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3061 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3121 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3181 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3241 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3301 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3361 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3421 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3481 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3541 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3601 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3661 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3721 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     3781 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     3841 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     3901 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     3961 tgcggcatca gagcagattg tactgagagt gcaccatatg gacatattgt cgttagaacg
     4021 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactata
//
