LOCUS       sequence-79099-e        5401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast marker swap plasmid for converting KanMX3 to ADE2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Voth WP, Jiang YW, Stillman DJ
  TITLE     New 'marker swap' plasmids for converting selectable markers on 
            budding yeast gene disruptions and plasmids.
  JOURNAL   Yeast. 2003 Aug;20(11):985-93.
  PUBMED    12898713
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2003-08"; volume: "20(11)"; pages: "985-93"
COMMENT     Sequence Label: M4753 KanMX::ADE2 Disruptor Converter
FEATURES             Location/Qualifiers
     source          1..5401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1260..1457
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1564..1583)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1565..1583)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1670..1687)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1841..2429)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1921..1940)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2600..3391,3392..3460))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3223..3242
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3461..3565)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3633..3651
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3689..3711)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3811..3830
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3911..3929
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3911..3928
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        4007..4350
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        4376..4751
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     CDS             join(4752..5401,1..1066)
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
ORIGIN
        1 tatttgtgac ttatgttatg cgcctgctag agttccggac tccgttcaac ttaaggcgaa
       61 gttgttggca gaaaatgcaa tcaaatcttt tcccggttgt ggtatatttg gtgtggaaat
      121 gttctattta gaaacagggg aattgcttat taacgaaatt gccccaaggc ctcacaactc
      181 tggacattat accattgatg cttgcgtcac ttctcaattt gaagctcatt tgagatcaat
      241 attggatttg ccaatgccaa agaatttcac atctttctcc accattacaa cgaacgccat
      301 tatgctaaat gttcttggag acaaacatac aaaagataaa gagctagaaa cttgcgaaag
      361 agcattggcg actccaggtt cctcagtgta cttatatgga aaagagtcta gacctaacag
      421 aaaagtaggt cacataaata ttattgcctc cagtatggcg gaatgtgaac aaaggctgaa
      481 ctacattaca ggtagaactg atattccaat caaaatctct gtcgctcaaa agttggactt
      541 ggaagcaatg gtcaaaccat tggttggaat catcatggga tcagactctg acttgccggt
      601 aatgtctgcc gcatgtgcgg ttttaaaaga ttttggcgtt ccatttgaag tgacaatagt
      661 ctctgctcat agaactccac ataggatgtc agcatatgct atttccgcaa gcaagcgtgg
      721 aattaaaaca attatcgctg gagctggtgg ggctgctcac ttgccaggta tggtggctgc
      781 aatgacacca cttcctgtca tcggtgtgcc cgtaaaaggt tcttgtctag atggagtaga
      841 ttctttacat tcaattgtgc aaatgcctag aggtgttcca gtagctaccg tcgctattaa
      901 taatagtacg aacgctgcgc tgttggctgt cagactgctt ggcgcttatg attcaagtta
      961 tacaacgaaa atggaacagt ttttattaaa gcaagaagaa gaagttcttg tcaaagcaca
     1021 aaagttagaa actgtcggtt acgaagctta tctagaaaac aagtaatata taagtttatt
     1081 gatatacttg tacagcaaat aattataaaa tgatatacct attttttagg ctttgttatg
     1141 attacatcaa atgtggactt catacataga aatcaacgct tacaggtgtc cttttttaag
     1201 aatttcatac ataagatcct ctagagtcga cctgcaggca tgccctgccc ctaatcagta
     1261 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
     1321 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
     1381 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
     1441 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtg tcgaaaacga
     1501 gctcgaattc atcgatgata tcagatccac tagtggccta tgcggccgcg gatctgccgg
     1561 tctccctata gtgagtcgta ttaatttcga taagccaggt taacctgcat taatgaatcg
     1621 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1681 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1741 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1801 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1861 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1921 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1981 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     2041 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2101 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2161 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2221 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2281 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2341 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2401 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2461 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2521 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2581 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2641 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2701 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2761 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2821 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2881 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2941 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3001 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3061 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3121 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3181 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3241 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3301 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3361 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3421 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3481 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3541 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     3601 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     3661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     3721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     3781 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     3841 accatatgga catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca
     3901 tacacatacg atttaggtga cactatagaa cgcggccgcc agctgaagct taaggcgcgc
     3961 cagatctgtt tagcttgcct cgtccccgcc gggtcacccg gccagcgaca tggaggccca
     4021 gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc
     4081 gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg
     4141 cacggcgcga agcaaaaatt acggctcctc gctgcagacc tgcgagcagg gaaacgctcc
     4201 cctcacagac gcgttgaatt gtccccacgc cgcgcccctg tagagaaata taaaaggtta
     4261 ggatttgcca ctgaggttct tctttcatat acttcctttt aaaatcttgc taggatacag
     4321 ttctcacatc acatccgaac ataaacaacc atgggtaccc ggggatctga attaattctt
     4381 gaataataca taacttttct taaaagaatc aaagacagat aaaatttaag agatattaaa
     4441 tattagtgag aagccgagaa ttttgtaaca ccaacataac actgacatct ttaacaactt
     4501 ttaattatga tacatttctt acgtcatgat tgattattac agctatgctg acaaatgact
     4561 cttgttgcat ggctacgaac cgggtaatac taagtgattg actcttgctg accttttatt
     4621 aagaactaaa tggacaatat tatggagcat ttcatgtata aattggtgcg taaaatcgtt
     4681 ggatctctct tctaagtaca tcctactata acaatcaaga aaaacaagaa aatcggacaa
     4741 aacaatcaag tatggattct agaacagttg gtatattagg agggggacaa ttgggacgta
     4801 tgattgttga ggcagcaaac aggctcaaca ttaagacggt aatactagat gctgaaaatt
     4861 ctcctgccaa acaaataagc aactccaatg accacgttaa tggctccttt tccaatcctc
     4921 ttgatatcga aaaactagct gaaaaatgtg atgtgctaac gattgagatt gagcatgttg
     4981 atgttcctac actaaagaat cttcaagtaa aacatcccaa attaaaaatt tacccttctc
     5041 cagaaacaat cagattgata caagacaaat atattcaaaa agagcattta atcaaaaatg
     5101 gtatagcagt tacccaaagt gttcctgtgg aacaagccag tgagacgtcc ctattgaatg
     5161 ttggaagaga tttgggtttt ccattcgtct tgaagtcgag gactttggca tacgatggaa
     5221 gaggtaactt cgttgtaaag aataaggaaa tgattccgga agctttggaa gtactgaagg
     5281 atcgtccttt gtacgccgaa aaatgggcac catttactaa agaattagca gtcatgattg
     5341 tgaggtctgt taacggttta gtgttttctt acccaattgt agagactatc cacaaggaca
     5401 a
//
