LOCUS       sequence-79102-e        6600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast marker swap plasmid for converting LYS2 to HIS3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Voth WP, Jiang YW, Stillman DJ
  TITLE     New 'marker swap' plasmids for converting selectable markers on 
            budding yeast gene disruptions and plasmids.
  JOURNAL   Yeast. 2003 Aug;20(11):985-93.
  PUBMED    12898713
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2003-08"; volume: "20(11)"; pages: "985-93"
COMMENT     Sequence Label: D588 lys2::HIS3 Disruptor Converter
FEATURES             Location/Qualifiers
     source          1..6600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2221..2408
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             2409..3068
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(3682..3921)
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     primer_bind     complement(3984..4000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3984..4000)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3997..4019)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4008..4024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4032..4038,4039..4056,4057..4062))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4077..4098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4215..4232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4386..4974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4466..4485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5145..5936,5937..6005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5768..5787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6006..6110)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6178..6196
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6234..6256)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6356..6375
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6569..6591
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6583..6600
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6584..6600
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccgg ggatccggtg attgattgag caagctcgat ttgtctcaac ctgctttgga
       61 agtggacctt gattccttca aaaagcaaag tattgttagc cgtctaaaca tgagtggcaa
      121 gtggcaatca tcaacgaaaa tcgaagcact tgtggaagaa ctatacaaac tgagaagcaa
      181 caagagaacg attaaatcca ttgtgttttc ccagtttacc agtatgctgg atctggtaga
      241 gtggagattg aaaagagctg gatttcaaac agtgaagctt cagggtagta tgtcaccgac
      301 gcaaagagat gaaaccatca agtatttcat gaacaacatt caatgcgagg ttttcttggt
      361 cagtttaaag gcgggcggtg ttgctttgaa tctttgtgaa gcttcgcaag tattcatttt
      421 agacccatgg tggaacccta gtgttgaatg gcaaagtggt gatagagttc atagaattgg
      481 tcagtatcga cctgtgaaga tcacaaggtt ttgcattgag gatagtatag aagcaagaat
      541 cattgaatta caggaaaaaa aggcaaatat gattcatgct acaataaacc aagatgaagc
      601 tgccattagc agactaacgc cagctgattt acagttctta ttcaataact aatattttat
      661 tctcttatta tatattattc tcggagtttt taagtgacat cacccgaaaa gaagctaagt
      721 ctttctccta attcatattt aattattgta catggacata tcatacgtaa tgctcaacct
      781 taagctgctg cggagcttcc acgagcacca gcacctgaag caactagact tatttgcgct
      841 tgagttagtt ctatacttgg cagtggaagt ttgtcattat gagttggtgg aggtaaaaat
      901 ccaaccttgt ttaaaaatgc aatatatata ccaacctctt ctggagtaac acctattcca
      961 ttagaccaat caacacctgt ccatgcggtg tctttcttta aagatgccac ggcgttccta
     1021 tcgtctagtt ccggagcttt ggtactttca ggtaagttgt ctaagaccat gtgtagcaaa
     1081 ggatacaacg cattttcttc attcctgtca ataacagacg cctccaatga tttcttccat
     1141 ttagaatagc tttcgatttc gacatcgtaa ccataatcgt gtaaagtata caagtagtct
     1201 ttgaataata ttcttgggtg acccgttact tgagcaacgg ccaattcatt ttctttggga
     1261 ggattcaaag acgtagcaac aacaacacga gcaacatgat ctactggaac catattcacg
     1321 gaattttcga tatctggaat cttacctaat tggactgaac ctttcaaaaa tctcaataag
     1381 aaatcatctg tgtttgaaga accattggca gaggcacctg ttacgtaacc tggtctgaca
     1441 atacacccac gtaggcccct ttcacctgca cgtctaatga tgtactcagc agcccatttg
     1501 gactgaccat atccaccagt gagcccgctt gcagagttca ttaaatcgtc tgattctaaa
     1561 atgcctggct tcccttcgct aacaagttta tctgacaaat taaagtagta ttcagtgtca
     1621 agagtagaag tggaggaaac aaagtcaaag aactttggct tgccgacggc ggctaagctc
     1681 ataacattga tagttgaaat aacatttgga tccctcaatt tggcatatgg ataaacccag
     1741 tgaactaacg caccattatg gataattata tcaactgtgt ttgccaaatc catccacttc
     1801 tcatctgaaa gaccaaattg gcttttagat aaatcgccta atacaacttt aatatttgag
     1861 gcaaattttt cgttccaagt accataggtg atacctgcct tttgtaatct tgcaaatgca
     1921 gcttcttcat ccttggccct gacgtgggca aacactttga aactgtagtt ctttggagaa
     1981 cgtcctaaca aatctgcaag gatgtaggag cccagaaatc ctgtgacacc ggtaacaaac
     2041 acattaattg ttgtttttcc ttcggcacta ttaggttcaa caaaatattc tcgagatccg
     2101 gtgattgatt gagcaagctt aggagtcact gccaggtatc gtttgaacac ggcattagtc
     2161 agggaagtca taacacagtc ctttcccgca attttctttt tctattactc ttggcctcct
     2221 ctagtacact ctatattttt ttatgcctcg gtaatgattt tcattttttt ttttccacct
     2281 agcggatgac tctttttttt tcttagcgat tggcattatc acataatgaa ttatacatta
     2341 tataaagtaa tgtgatttct tcgaagaata tactaaaaaa tgagcaggca agataaacga
     2401 aggcaaagat gacagagcag aaagccctag taaagcgtat tacaaatgaa accaagattc
     2461 agattgcgat ctctttaaag ggtggtcccc tagcgataga gcactcgatc ttcccagaaa
     2521 aagaggcaga agcagtagca gaacaggcca cacaatcgca agtgattaac gtccacacag
     2581 gtatagggtt tctggaccat atgatacatg ctctggccaa gcattccggc tggtcgctaa
     2641 tcgttgagtg cattggtgac ttacacatag acgaccatca caccactgaa gactgcggga
     2701 ttgctctcgg tcaagctttt aaagaggccc tactggcgcg tggagtaaaa aggtttggat
     2761 caggatttgc gcctttggat gaggcacttt ccagagcggt ggtagatctt tcgaacaggc
     2821 cgtacgcagt tgtcgaactt ggtttgcaaa gggagaaagt aggagatctc tcttgcgaga
     2881 tgatcccgca ttttcttgaa agctttgcag aggctagcag aattaccctc cacgttgatt
     2941 gtctgcgagg caagaatgat catcaccgta gtgagagtgc gttcaaggct cttgcggttg
     3001 ccataagaga agccacctcg cccaatggta ccaacgatgt tccctccacc aaaggtgttc
     3061 ttatgtagtg acaccgatta tttaaagctg cagcatacga tatatataca tgtgtatata
     3121 tgtataccta tgaatgtcag taagtatgta tacgaacagt atgatactga agatgacaag
     3181 gtaatgcatc attctatacg tgtcattctg aacgaggcgc gctttccttt tttctttttg
     3241 ctttttcttt ttttttctct tgaactcgaa gcttgctcaa tcaatcaccg gatcttggca
     3301 actttgaatt ttttcagcta gctcgtcaaa ggaaaaattg gcctcaatag aattgagctt
     3361 gttcaactca ttgttaattg tagaatacag ctcattaaat gaccacgttg gttgaatatt
     3421 gaatcttaag attttgttat tcgcaatata aagaacaata tcatcgtcac cggttactct
     3481 atatatcaat gcagcccata cactcaaagc gacagcgtat ttgttagaaa aactatcatg
     3541 aggcacatcg agctgaggta gctgtaacga atatgtagct tgtttcgtat aaggttcttg
     3601 ttgtgggcgt aaaaagtcat gtggtaacac tgaaagagtt ggattatcca acttctctat
     3661 ccagaccttt tcgttagtca ttagtgagta actctgtgat atctctctat aattagcagt
     3721 ttttcactga aattccagga aaggtaataa actcagattt ttttttatac tattggctgc
     3781 ttgttactta tatatcttga acttctccca gcgggtcttc aaatacattt gggcgatgtt
     3841 catgttcatt aggcaggtat ttcgacattg agtcacacgc gaaaaaccgc cggaattttt
     3901 tatgtaattg caagtggaat tagcttgctc aatcaatcac cggatccgtc gacctgcagc
     3961 caagctagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4021 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4081 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4141 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4201 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4261 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4321 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4381 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4441 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4501 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4561 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4621 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4681 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4741 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4801 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     4861 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4921 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4981 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5041 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5101 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5161 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5221 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5281 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5341 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5401 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5461 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5521 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5581 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5641 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5701 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5761 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5821 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5881 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5941 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6001 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6061 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6121 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     6181 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     6241 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     6301 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     6361 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     6421 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     6481 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     6541 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
//
