LOCUS       sequence-79103-e        9016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast marker swap plasmid for converting HIS3 to LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Voth WP, Jiang YW, Stillman DJ
  TITLE     New 'marker swap' plasmids for converting selectable markers on 
            budding yeast gene disruptions and plasmids.
  JOURNAL   Yeast. 2003 Aug;20(11):985-93.
  PUBMED    12898713
REFERENCE   2  (bases 1 to 9016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2003-08"; volume: "20(11)"; pages: "985-93"
COMMENT     Sequence Label: D1433 his3::LYS2 Disruptor Converter
FEATURES             Location/Qualifiers
     source          1..9016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        138..325
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             complement(1493..5671)
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        complement(5672..5911)
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     primer_bind     complement(6400..6416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6400..6416)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6413..6435)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6424..6440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6448..6454,6455..6472,6473..6478))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6493..6514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6631..6648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6802..7390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6882..6901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7561..8352,8353..8421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8184..8203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8422..8526)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8594..8612
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8650..8672)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8772..8791
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8985..9007
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8999..9016
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9000..9016
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccgg ggatccggtg attgattgag caagcttagg agtcactgcc aggtatcgtt
       61 tgaacacggc attagtcagg gaagtcataa cacagtcctt tcccgcaatt ttctttttct
      121 attactcttg gcctcctcta gtacactcta tattttttta tgcctcggta atgattttca
      181 tttttttttt tccacctagc ggatgactct ttttttttct tagcgattgg cattatcaca
      241 taatgaatta tacattatat aaagtaatgt gatttcttcg aagaatatac taaaaaatga
      301 gcaggcaaga taaacgaagg caaagatgac agagcagaaa gccctagtaa agcgtattac
      361 aaatgaaacc aagattcaga ttgcgatctc tttaaagggt ggtcccctag cgatagagca
      421 ctcgatcttc ccagaaaaag aggcagaagc agtagcagaa caggccacac aatcgcaagt
      481 gattaacgtc cacacaggta tagggtttct ggaccatatg atacatgctc tggccaagca
      541 ttccggctgg tcgctaatcg ttgagtgcat tggtgactta cacatagacg accatcacac
      601 cactgaagac tgcgggattg ctctcggtca agcttttaaa gaggccctag gggccgtgcg
      661 tggagtaaaa aggtttggat caggatttgc gcctttggat gaggcacttt ccagagcggt
      721 ggtagatccg gtgattgatt gagcaagctc gatttgtctc aacctgcttt ggaagtggac
      781 cttgattcct tcaaaaagca aagtattgtt agccgtctaa acatgagtgg caagtggcaa
      841 tcatcaacga aaatcgaagc acttgtggaa gaactataca aactgagaag caacaagaga
      901 acgattaaat ccattgtgtt ttcccagttt accagtatgc tggatctggt agagtggaga
      961 ttgaaaagag ctggatttca aacagtgaag cttcagggta gtatgtcacc gacgcaaaga
     1021 gatgaaacca tcaagtattt catgaacaac attcaatgcg aggttttctt ggtcagttta
     1081 aaggcgggcg gtgttgcttt gaatctttgt gaagcttcgc aagtattcat tttagaccca
     1141 tggtggaacc ctagtgttga atggcaaagt ggtgatagag ttcatagaat tggtcagtat
     1201 cgacctgtga agatcacaag gttttgcatt gaggatagta tagaagcaag aatcattgaa
     1261 ttacaggaaa aaaaggcaaa tatgattcat gctacaataa accaagatga agctgccatt
     1321 agcagactaa cgccagctga tttacagttc ttattcaata actaatattt tattctctta
     1381 ttatatatta ttctcggagt ttttaagtga catcacccga aaagaagcta agtctttctc
     1441 ctaattcata tttaattatt gtacatggac atatcatacg taatgctcaa ccttaagctg
     1501 ctgcggagct tccacgagca ccagcacctg aagcaactag acttatttgc gcttgagtta
     1561 gttctatact tggcagtgga agtttgtcat tatgagttgg tggaggtaaa aatccaacct
     1621 tgtttaaaaa tgcaatatat ataccaacct cttctggagt aacacctatt ccattagacc
     1681 aatcaacacc tgtccatgcg gtgtctttct ttaaagatgc cacggcgttc ctatcgtcta
     1741 gttccggagc tttggtactt tcaggtaagt tgtctaagac catgtgtagc aaaggataca
     1801 acgcattttc ttcattcctg tcaataacag acgcctccaa tgatttcttc catttagaat
     1861 agctttcgat ttcgacatcg taaccataat cgtgtaaagt atacaagtag tctttgaata
     1921 atattcttgg gtgacccgtt acttgagcaa cggccaattc attttctttg ggaggattca
     1981 aagacgtagc aacaacaaca cgagcaacat gatctactgg aaccatattc acggaatttt
     2041 cgatatctgg aatcttacct aattggactg aacctttcaa aaatctcaat aagaaatcat
     2101 ctgtgtttga agaaccattg gcagaggcac ctgttacgta acctggtctg acaatacacc
     2161 cacgtaggcc cctttcacct gcacgtctaa tgatgtactc agcagcccat ttggactgac
     2221 catatccacc agtgagcccg cttgcagagt tcattaaatc gtctgattct aaaatgcctg
     2281 gcttcccttc gctaacaagt ttatctgaca aattaaagta gtattcagtg tcaagagtag
     2341 aagtggagga aacaaagtca aagaactttg gcttgccgac ggcggctaag ctcataacat
     2401 tgatagttga aataacattt ggatccctca atttggcata tggataaacc cagtgaacta
     2461 acgcaccatt atggataatt atatcaactg tgtttgccaa atccatccac ttctcatctg
     2521 aaagaccaaa ttggctttta gataaatcgc ctaatacaac tttaatattt gaggcaaatt
     2581 tttcgttcca agtaccatag gtgatacctg ccttttgtaa tcttgcaaat gcagcttctt
     2641 catccttggc cctgacgtgg gcaaacactt tgaaactgta gttctttgga gaacgtccta
     2701 acaaatctgc aaggatgtag gagcccagaa atcctgtgac accggtaaca aacacattaa
     2761 ttgttgtttt tccttcggca ctattaggtt caacaaaata ttctcgagag gggtacgaac
     2821 ttggtagcgt ctcaaccaat ttcttggcgt cttccgcata atttgcagtg acattttcga
     2881 cgacctcacc ttgagatgat ccacccgatg atttaattct gtcaatttcc gcggcaaagg
     2941 cctttatcgt tggatacttg aaaattgtgc ccaatggtaa atcaacttgc agctttttct
     3001 ttaaggtaaa aatcattttg gtagccaaga tagaatgacc acctaaatcg aaaaacgaat
     3061 catctggtga tacagatgct ggcttggtag gtaatatact taaccataag tctctaacct
     3121 cgcgctcaac attggtaaac tgagagtcgt cagtttcaga aactgtattt tcagctacca
     3181 aatttaattg cttgggagtt gggaattgaa gtttaggctt atcaacttta ccatttggat
     3241 tcaatggtag tttatccata accacaatca aggaaggcat agcatagcta gccaatcttt
     3301 tctttaagaa agtcctgatg tccttggata aaagatggta accgattaag cccttaacta
     3361 tagggtcagt ttcaacctcc tttggaacat cactttggaa cttagacaag tcatctggct
     3421 tgtcaaatct tgggaccata aatgtgatca atgttggctc attgtcggca tttttgcgaa
     3481 ctaaagtaat gttttctctt accaatggat gttgggaaat gtgcgtatct atttctccta
     3541 attcgattct gaacccacga attttaactt gatcatcagc cctaccgcaa cattcacagt
     3601 caccgtttgg tagataacga cctaaatcac ccgttctgta caatctatct cttggaccta
     3661 accagaattg tctccaaggt tcaccattat ccttatccaa ataattccag tgatcttttt
     3721 caacaaacca gttgttcaca aatttttctt tattcaattc tggtaatcct ctataacctt
     3781 cggccaaacc acctgcacga acataaatct cacctatttc gccaatacca catatttgag
     3841 tacgatcgtt cctgttaaca actagtagct gaacgttcaa cataccttta ccagcaggca
     3901 tgacatcttt caattttttc aaaaagtttg gatcgtcatt ttttgattta acttcgaaat
     3961 aagaaactgc acgctgtgtt tcagtggtac cgtacatatt aacaatacgg caattttctg
     4021 ccaaggtttg taacctcaga caatcacgtt ttgttaaaat gtcacccaca aagaacgcat
     4081 gatgtaactt agggaatggt gtagtagctt gggcagtaag taattgaccc atggcaggtg
     4141 ttaaatgggt aactgtgcaa ccatacttac tcatccattc cgctaaacgg cccggtgtac
     4201 caatatcatc ttgagtaggg acatacaatt gggcacctaa aaataatggt gtaaacatat
     4261 ctctttgaat tggatcatgt gcaataccgc tcagcattgt gaatttatca ttttctgtta
     4321 agttgaacct tttggacatc caattgaaat aataagccaa ggaaaaatgt ctaccaagaa
     4381 cacccttagg aataccttcg gaaccagatg tgaaagatag ggttgggttg gaatctggtc
     4441 caactacaac acctgttctg gtgtctttgt agtgatcata tggagccaaa acatcctcgc
     4501 cattgtccaa tttgccacct tcaatggtac cattttcttg aatagcgatg gaattgattc
     4561 ttgaaacaat ctccaattca tcattgatgt aatcttctac tagttgatcc aattgtccag
     4621 cagctctaat aacaatcaac ccacgtggtt tagcaacacc taagtaaatg gtttgtctgg
     4681 ctgggggata tgcagggtcg ataactgaaa aggttgcgcc ggctttcaag acacccatca
     4741 cacataccat caaatccaca cccctagaag aatagatcat cactacatca cctcttttga
     4801 tacctgtttt aatcaaataa tgggcaacta tgttagaagt gcggttgatg tcgcgataag
     4861 tgaaagaacg ggacttgtcg gaatttagtg ttggagtctc cacaacacag gttctctctg
     4921 ggaaggcttc agcattgtcc tggaaaatgt cgtgaataca ccccacgaaa tcgcaccagc
     4981 ccaagttctt agttggatca ggtaaactat ccttggatga tgcggtgatc agagagattt
     5041 tagttatgca attggatgga tcgcttagcg cagcagtcaa atattgagta aattggtccg
     5101 caacaatggt tactctttca ttcgaataca gtaagctgtt ataaattaag ttcaaaacat
     5161 gcgcattatt actggtatcc aaattcaaag caaagtccac taaatgatgc ttgaactcgt
     5221 ctaatttgaa atcttggttt tccaaaaagg ccaaacggaa caactgaggg gtcctttcca
     5281 gatcttggca actttgaatt ttttcagcta gctcgtcaaa ggaaaaattg gcctcaatag
     5341 aattgagctt gttcaactca ttgttaattg tagaatacag ctcattaaat gaccacgttg
     5401 gttgaatatt gaatcttaag attttgttat tcgcaatata aagaacaata tcatcgtcac
     5461 cggttactct atatatcaat gcagcccata cactcaaagc gacagcgtat ttgttagaaa
     5521 aactatcatg aggcacatcg agctgaggta gctgtaacga atatgtagct tgtttcgtat
     5581 aaggttcttg ttgtgggcgt aaaaagtcat gtggtaacac tgaaagagtt ggattatcca
     5641 acttctctat ccagaccttt tcgttagtca ttagtgagta actctgtgat atctctctat
     5701 aattagcagt ttttcactga aattccagga aaggtaataa actcagattt ttttttatac
     5761 tattggctgc ttgttactta tatatcttga acttctccca gcgggtcttc aaatacattt
     5821 gggcgatgtt catgttcatt aggcaggtat ttcgacattg agtcacacgc gaaaaaccgc
     5881 cggaattttt tatgtaattg caagtggaat tagcttgctc aatcaatcac cggatctctc
     5941 ttgcgagatg atcccgcatt ttcttgaaag ctttgcagag gctagcagaa ttaccctcca
     6001 cgttgattgt ctgcgaggca agaatgatca tcaccgtagt gagagtgcgt tcaaggctct
     6061 tgcggttgcc ataagagaag ccacctcgcc caatggtacc aacgatgttc cctccaccaa
     6121 aggtgttctt atgtagtgac accgattatt taaagctgca gcatacgata tatatacatg
     6181 tgtatatatg tatacctatg aatgtcagta agtatgtata cgaacagtat gatactgaag
     6241 atgacaaggt aatgcatcat tctatacgtg tcattctgaa cgaggcgcgc tttccttttt
     6301 tctttttgct ttttcttttt ttttctcttg aactcgaagc ttgctcaatc aatcaccgga
     6361 tccgtcgacc tgcagccaag ctagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6421 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6481 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6541 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6601 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6661 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7201 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     7261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8521 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     8581 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     8641 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     8701 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     8761 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     8821 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     8881 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     8941 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     9001 taaaacgacg gccagt
//
