LOCUS       sequence-79105-e        8048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast marker swap plasmid for converting LEU2 to ADE2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Voth WP, Jiang YW, Stillman DJ
  TITLE     New 'marker swap' plasmids for converting selectable markers on 
            budding yeast gene disruptions and plasmids.
  JOURNAL   Yeast. 2003 Aug;20(11):985-93.
  PUBMED    12898713
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2003-08"; volume: "20(11)"; pages: "985-93"
COMMENT     Sequence Label: M3939 leu2::ADE2 Disruptor Converter
FEATURES             Location/Qualifiers
     source          1..8048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..442
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        2105..2480
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     CDS             2481..4196
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     CDS             complement(4495..4506)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(5432..5448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5432..5448)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5445..5467)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5456..5472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5480..5486,5487..5504,5505..5510))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5525..5546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5663..5680)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5834..6422)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5914..5933)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6593..7384,7385..7453))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7216..7235
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7454..7558)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7626..7644
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7682..7704)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7804..7823
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8017..8039
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8031..8048
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8032..8048
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccgg ggatccggtg attgattgag caagctaact gtgggaatac tcaggtatcg
       61 taagatgcaa gagttcgaat ctcttagcaa ccattatttt tttcctcaac ataacgagaa
      121 cacacagggg cgctatcgca cagaatcaaa ttcgatgact ggaaattttt tgttaatttc
      181 agaggtcgcc tgacgcatat acctttttca actgaaaaat tgggagaaaa aggaaaggtg
      241 agagcgccgg aaccggcttt tcatatagaa tagagaagcg ttcatgacta aatgcttgca
      301 tcacaatact tgaagttgac aatattattt aaggacctat tgttttttcc aataggtggt
      361 tagcaatcgt cttactttct aacttttctt accttttaca tttcagcaat atatatatat
      421 atttcaagga tataccattc taatgtctgc ccctaagaag atcgtcgttt tgccaggtga
      481 ccacgttggt caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt
      541 tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc
      601 tacaggtgtt ccacttccag atgaggcgct ggaagcctcc aagaaggctg atgccgtttt
      661 gttaggtgct gtgggtggtc ctaaatgggg taccggtagt gttagacctg aacaaggttt
      721 actaaaaatc cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc
      781 cgactctctt ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt
      841 tgttgtcaga gaattagtgg gaggtattta ctttggtaag agaaaggaag acgatggtga
      901 tggtgtcgct tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat
      961 ggccgctttc atggccctac aacatgagcc accattgcct atttggtcct tggataaagc
     1021 taatgttttg gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga
     1081 attccctaca ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa
     1141 gaacccaacc cacctaaatg gtattataat caccagcaac atgtttggtg atgatcccga
     1201 tctttttact caaatttaca gttttgatat cttggcgttt ttcctcacat ttattacaaa
     1261 agaaagtggg caggatttgc ccttcacaaa atctctgtcc aggagaagaa cgtatttgac
     1321 cagaattgct caatcttctt cattgagaca gtttttacaa ctgagtacac gccctaatat
     1381 aaggattctg ggaggtaatg ggcagggtac tggaaattcc aattatcccg aatttacaaa
     1441 cgcatcctca gtcatctcac ccaagctaag ggcttctcct ctactagaaa gaagggcgtc
     1501 aaaaatctgc tatgctatta cgaaattact ctataggtta gaatgtcatc ccataggtat
     1561 ggccctttta aagaagtatc tccacaatca attgcgagaa gcctatctag aatcaaaacg
     1621 acactttatt tccaaaaagg gagattcgac caacacttcc tctaccattg catcatcatc
     1681 tttcgctggc gcatctgttc ctctatcttc aaacgaatca ggaatgctaa acggcttgaa
     1741 gcaaattaac gaacaacaag aatctacatt agaaaccact caaaaggaag actagtaacg
     1801 ccgtatcgtg attaacgtat tacataagtt acaggattca tgcttatggg ttagctattt
     1861 cgcccaatgt gtccatctga cattactatt ttgcatttta atttaattag aacttgacta
     1921 gcgcactacc agtatatcat ctcatttccg taaataccaa atgtattata tattgaaagc
     1981 ttttgaccag gttattataa aagaaacttc atgctcgaaa aagatcattt cgaaaagttg
     2041 cctagtttca tgaaatttta aagcagttta tataaatttt accttttgat gcggaattga
     2101 ctttttcttg aataatacat aacttttctt aaaagaatca aagacagata aaatttaaga
     2161 gatattaaat attagtgaga agccgagaat tttgtaacac caacataaca ctgacatctt
     2221 taacaacttt taattatgat acatttctta cgtcatgatt gattattaca gctatgctga
     2281 caaatgactc ttgttgcatg gctacgaacc gggtaatact aagtgattga ctcttgctga
     2341 ccttttatta agaactaaat ggacaatatt atggagcatt tcatgtataa attggtgcgt
     2401 aaaatcgttg gatctctctt ctaagtacat cctactataa caatcaagaa aaacaagaaa
     2461 atcggacaaa acaatcaagt atggattcta gaacagttgg tatattagga gggggacaat
     2521 tgggacgtat gattgttgag gcagcaaaca ggctcaacat taagacggta atactagatg
     2581 ctgaaaattc tcctgccaaa caaataagca actccaatga ccacgttaat ggctcctttt
     2641 ccaatcctct tgatatcgaa aaactagctg aaaaatgtga tgtgctaacg attgagattg
     2701 agcatgttga tgttcctaca ctaaagaatc ttcaagtaaa acatcccaaa ttaaaaattt
     2761 acccttctcc agaaacaatc agattgatac aagacaaata tattcaaaaa gagcatttaa
     2821 tcaaaaatgg tatagcagtt acccaaagtg ttcctgtgga acaagccagt gagacgtccc
     2881 tattgaatgt tggaagagat ttgggttttc cattcgtctt gaagtcgagg actttggcat
     2941 acgatggaag aggtaacttc gttgtaaaga ataaggaaat gattccggaa gctttggaag
     3001 tactgaagga tcgtcctttg tacgccgaaa aatgggcacc atttactaaa gaattagcag
     3061 tcatgattgt gagatctgtt aacggtttag tgttttctta cccaattgta gagactatcc
     3121 acaaggacaa tatttgtgac ttatgttatg cgcctgctag agttccggac tccgttcaac
     3181 ttaaggcgaa gttgttggca gaaaatgcaa tcaaatcttt tcccggttgt ggtatatttg
     3241 gtgtggaaat gttctattta gaaacagggg aattgcttat taacgaaatt gccccaaggc
     3301 ctcacaactc tggacattat accattgatg cttgcgtcac ttctcaattt gaagctcatt
     3361 tgagatcaat attggatttg ccaatgccaa agaatttcac atctttctcc accattacaa
     3421 cgaacgccat tatgctaaat gttcttggag acaaacatac aaaagataaa gagctagaaa
     3481 cttgcgaaag agcattggcg actccaggtt cctcagtgta cttatatgga aaagagtcta
     3541 gacctaacag aaaagtaggt cacataaata ttattgcctc cagtatggcg gaatgtgaac
     3601 aaaggctgaa ctacattaca ggtagaactg atattccaat caaaatctct gtcgctcaaa
     3661 agttggactt ggaagcaatg gtcaaaccat tggttggaat catcatggga tcagactctg
     3721 acttgccggt aatgtctgcc gcatgtgcgg ttttaaaaga ttttggcgtt ccatttgaag
     3781 tgacaatagt ctctgctcat agaactccac ataggatgtc agcatatgct atttccgcaa
     3841 gcaagcgtgg aattaaaaca attatcgctg gagctggtgg ggctgctcac ttgccaggta
     3901 tggtggctgc aatgacacca cttcctgtca tcggtgtgcc cgtaaaaggt tcttgtctag
     3961 atggagtaga ttctttacat tcaattgtgc aaatgcctag aggtgttcca gtagctaccg
     4021 tcgctattaa taatagtacg aacgctgcgc tgttggctgt cagactgctt ggcgcttatg
     4081 attcaagtta tacaacgaaa atggaacagt ttttattaaa gcaagaagaa gaagttcttg
     4141 tcaaagcaca aaagttagaa actgtcggtt acgaagctta tctagaaaac aagtaatata
     4201 taagtttatt gatatacttg tacagcaaat aattataaaa tgatatacct attttttagg
     4261 ctttgttatg attacatcaa atgtggactt catacataga aatcaacgct tacaggtgtc
     4321 cttttttaag aatttcatac ataagatcac ttattataca tacatacata tccagtaaca
     4381 agaagcaagg aataattacc tgcttaagtc tgcgattaaa aaaataacgt ttcgatacag
     4441 ttcatataag gcggctcaat gcagaaccga ggatagcgct acgtcaggat atctttgtag
     4501 ttcccaaata taaatgcgac aatatagttt ctttctttca tatcaataat atccttttct
     4561 ccactgaaat cacgaatcaa acctggagca aaaactaaag ccaaattata aagcgtcatt
     4621 cgattccagt gactgtaccg tgtaaccttt tctatatgtt cactcagtac tcttaacacc
     4681 ctataatgtt cccttggaag gtcttccaat atgtttttta aagcgctctt gctcgacata
     4741 taagtgtccg aattctttgc ttctaaggac aactttcctc caacaaatgg caagttttcc
     4801 atcatttttt tagatttaac taacctcatc aacggctcgt atatttgaaa ggtaaagata
     4861 gggttgggga gctttcttaa gtatcgcttc aacacaccag taacaacgtt gagatcccgg
     4921 atcatcatct ccgatgaagc ctccgttatc ccaggttcct tgggtttgtt gccatctgcg
     4981 tccttggcct ctttgccaga caagaacacc gcatttggtt tgtacgaacc atgccacggt
     5041 tctgctccag atttgccaaa gaataaggtc aaccctatcg ccactatctt gtctgctgca
     5101 atgatgttga aattgtcatt gaacttgcct gaagaaggta aggccattga agatgcagtt
     5161 aaaaaggttt tggatgcagg tatcagaact ggtgatttag gtggttccaa cagtaccacc
     5221 gaagtcggtg atgctgtcgc cgaagaagtt aagaaaatcc ttgcttaaaa agattctctt
     5281 tttttatgat atttgtacat aaactttata aatgaaattc ataatagaaa cgacacgaaa
     5341 ttacaaaatg gaatatgttc atagggtaga gcttgctcaa tcaatcaccg gatccgtcga
     5401 cctgcagcca agctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5461 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5521 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5581 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5641 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5701 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5761 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5821 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5881 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5941 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6001 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6061 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6121 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6181 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6241 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6301 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6361 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6421 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6481 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6541 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6601 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6661 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6721 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6781 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6841 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6901 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6961 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7021 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7081 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7141 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7201 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7261 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7321 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7381 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7441 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7501 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7561 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     7621 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     7681 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7741 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     7801 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7861 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     7921 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     7981 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     8041 cggccagt
//
