LOCUS       sequence-79108-e        8206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast marker swap plasmid for converting URA3 to LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Voth WP, Jiang YW, Stillman DJ
  TITLE     New 'marker swap' plasmids for converting selectable markers on 
            budding yeast gene disruptions and plasmids.
  JOURNAL   Yeast. 2003 Aug;20(11):985-93.
  PUBMED    12898713
REFERENCE   2  (bases 1 to 8206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2003-08"; volume: "20(11)"; pages: "985-93"
COMMENT     Sequence Label: M2660 ura3::LYS2 Disruptor Converter
FEATURES             Location/Qualifiers
     source          1..8206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        642..881
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             882..5060
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(5985..6001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5985..6001)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5998..6020)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6009..6025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6033..6039,6040..6057,6058..6063))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6078..6099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6216..6233)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6387..6975)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6467..6486)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7146..7937,7938..8006))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7769..7788
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8007..8111)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8179..8197
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttttcaat tcaattcatc
      421 attttttttt tattcttttt tttgatttcg gtttccttga aatttttttg attcggtaat
      481 ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat
      541 atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa
      601 aacctgcagg tcgacggatc cggtgattga ttgagcaagc taattccact tgcaattaca
      661 taaaaaattc cggcggtttt tcgcgtgtga ctcaatgtcg aaatacctgc ctaatgaaca
      721 tgaacatcgc ccaaatgtat ttgaagaccc gctgggagaa gttcaagata tataagtaac
      781 aagcagccaa tagtataaaa aaaaatctga gtttattacc tttcctggaa tttcagtgaa
      841 aaactgctaa ttatagagag atatcacaga gttactcact aatgactaac gaaaaggtct
      901 ggatagagaa gttggataat ccaactcttt cagtgttacc acatgacttt ttacgcccac
      961 aacaagaacc ttatacgaaa caagctacat attcgttaca gctacctcag ctcgatgtgc
     1021 ctcatgatag tttttctaac aaatacgctg tcgctttgag tgtatgggct gcattgatat
     1081 atagagtaac cggtgacgat gatattgttc tttatattgc gaataacaaa atcttaagat
     1141 tcaatattca accaacgtgg tcatttaatg agctgtattc tacaattaac aatgagttga
     1201 acaagctcaa ttctattgag gccaattttt cctttgacga gctagctgaa aaaattcaaa
     1261 gttgccaaga tctggaaagg acccctcagt tgttccgttt ggcctttttg gaaaaccaag
     1321 atttcaaatt agacgagttc aagcatcatt tagtggactt tgctttgaat ttggatacca
     1381 gtaataatgc gcatgttttg aacttaattt ataacagctt actgtattcg aatgaaagag
     1441 taaccattgt tgcggaccaa tttactcaat atttgactgc tgcgctaagc gatccatcca
     1501 attgcataac taaaatctct ctgatcaccg catcatccaa ggatagttta cctgatccaa
     1561 ctaagaactt gggctggtgc gatttcgtgg ggtgtattca cgacattttc caggacaatg
     1621 ctgaagcctt cccagagaga acctgtgttg tggagactcc aacactaaat tccgacaagt
     1681 cccgttcttt cacttatcgc gacatcaacc gcacttctaa catagttgcc cattatttga
     1741 ttaaaacagg tatcaaaaga ggtgatgtag tgatgatcta ttcttctagg ggtgtggatt
     1801 tgatggtatg tgtgatgggt gtcttgaaag ccggcgcaac cttttcagtt atcgaccctg
     1861 catatccccc agccagacaa accatttact taggtgttgc taaaccacgt gggttgattg
     1921 ttattagagc tgctggacaa ttggatcaac tagtagaaga ttacatcaat gatgaattgg
     1981 agattgtttc aagaatcaat tccatcgcta ttcaagaaaa tggtaccatt gaaggtggca
     2041 aattggacaa tggcgaggat gttttggctc catatgatca ctacaaagac accagaacag
     2101 gtgttgtagt tggaccagat tccaacccaa ccctatcttt cacatctggt tccgaaggta
     2161 ttcctaaggg tgttcttggt agacattttt ccttggctta ttatttcaat tggatgtcca
     2221 aaaggttcaa cttaacagaa aatgataaat tcacaatgct gagcggtatt gcacatgatc
     2281 caattcaaag agatatgttt acaccattat ttttaggtgc ccaattgtat gtccctactc
     2341 aagatgatat tggtacaccg ggccgtttag cggaatggat gagtaagtat ggttgcacag
     2401 ttacccattt aacacctgcc atgggtcaat tacttactgc ccaagctact acaccattcc
     2461 ctaagttaca tcatgcgttc tttgtgggtg acattttaac aaaacgtgat tgtctgaggt
     2521 tacaaacctt ggcagaaaat tgccgtattg ttaatatgta cggtaccact gaaacacagc
     2581 gtgcagtttc ttatttcgaa gttaaatcaa aaaatgacga tccaaacttt ttgaaaaaat
     2641 tgaaagatgt catgcctgct ggtaaaggta tgttgaacgt tcagctacta gttgttaaca
     2701 ggaacgatcg tactcaaata tgtggtattg gcgaaatagg tgagatttat gttcgtgcag
     2761 gtggtttggc cgaaggttat agaggattac cagaattgaa taaagaaaaa tttgtgaaca
     2821 actggtttgt tgaaaaagat cactggaatt atttggataa ggataatggt gaaccttgga
     2881 gacaattctg gttaggtcca agagatagat tgtacagaac gggtgattta ggtcgttatc
     2941 taccaaacgg tgactgtgaa tgttgcggta gggctgatga tcaagttaaa attcgtgggt
     3001 tcagaatcga attaggagaa atagatacgc acatttccca acatccattg gtaagagaaa
     3061 acattacttt agttcgcaaa aatgccgaca atgagccaac attgatcaca tttatggtcc
     3121 caagatttga caagccagat gacttgtcta agttccaaag tgatgttcca aaggaggttg
     3181 aaactgaccc tatagttaag ggcttaatcg gttaccatct tttatccaag gacatcagga
     3241 ctttcttaaa gaaaagattg gctagctatg ctatgccttc cttgattgtg gttatggata
     3301 aactaccatt gaatccaaat ggtaaagttg ataagcctaa acttcaattc ccaactccca
     3361 agcaattaaa tttggtagct gaaaatacag tttctgaaac tgacgactct cagtttacca
     3421 atgttgagcg cgaggttaga gacttatggt taagtatatt acctaccaag ccagcatctg
     3481 tatcaccaga tgattcgttt ttcgatttag gtggtcattc tatcttggct accaaaatga
     3541 tttttacctt aaagaaaaag ctgcaagttg atttaccatt gggcacaatt ttcaagtatc
     3601 caacgataaa ggcctttgcc gcggaaattg acagaattaa atcatcgggt ggatcatctc
     3661 aaggtgaggt cgtcgaaaat gtcactgcaa attatgcgga agacgccaag aaattggttg
     3721 agacgctacc aagttcgtac ccctctcgag aatattttgt tgaacctaat agtgccgaag
     3781 gaaaaacaac aattaatgtg tttgttaccg gtgtcacagg atttctgggc tcctacatcc
     3841 ttgcagattt gttaggacgt tctccaaaga actacagttt caaagtgttt gcccacgtca
     3901 gggccaagga tgaagaagct gcatttgcaa gattacaaaa ggcaggtatc acctatggta
     3961 cttggaacga aaaatttgcc tcaaatatta aagttgtatt aggcgattta tctaaaagcc
     4021 aatttggtct ttcagatgag aagtggatgg atttggcaaa cacagttgat ataattatcc
     4081 ataatggtgc gttagttcac tgggtttatc catatgccaa attgagggat ccaaatgtta
     4141 tttcaactat caatgttatg agcttagccg ccgtcggcaa gccaaagttc tttgactttg
     4201 tttcctccac ttctactctt gacactgaat actactttaa tttgtcagat aaacttgtta
     4261 gcgaagggaa gccaggcatt ttagaatcag acgatttaat gaactctgca agcgggctca
     4321 ctggtggata tggtcagtcc aaatgggctg ctgagtacat cattagacgt gcaggtgaaa
     4381 ggggcctacg tgggtgtatt gtcagaccag gttacgtaac aggtgcctct gccaatggtt
     4441 cttcaaacac agatgatttc ttattgagat ttttgaaagg ttcagtccaa ttaggtaaga
     4501 ttccagatat cgaaaattcc gtgaatatgg ttccagtaga tcatgttgct cgtgttgttg
     4561 ttgctacgtc tttgaatcct cccaaagaaa atgaattggc cgttgctcaa gtaacgggtc
     4621 acccaagaat attattcaaa gactacttgt atactttaca cgattatggt tacgatgtcg
     4681 aaatcgaaag ctattctaaa tggaagaaat cattggaggc gtctgttatt gacaggaatg
     4741 aagaaaatgc gttgtatcct ttgctacaca tggtcttaga caacttacct gaaagtacca
     4801 aagctccgga actagacgat aggaacgccg tggcatcttt aaagaaagac accgcatgga
     4861 caggtgttga ttggtctaat ggaataggtg ttactccaga agaggttggt atatatattg
     4921 catttttaaa caaggttgga tttttacctc caccaactca taatgacaaa cttccactgc
     4981 caagtataga actaactcaa gcgcaaataa gtctagttgc ttcaggtgct ggtgctcgtg
     5041 gaagctccgc agcagcttaa ggttgagcat tacgtatgat atgtccatgt acaataatta
     5101 aatatgaatt aggagaaaga cttagcttct tttcgggtga tgtcacttaa aaactccgag
     5161 aataatatat aataagagaa taaaatatta gttattgaat aagaactgta aatcagatct
     5221 tgactgattt ttccatggag ggcacagtta agccgctaaa ggcattatcc gccaagtaca
     5281 attttttact cttcgaagac agaaaatttg ctgacattgg taatacagtc aaattgcagt
     5341 actctgcggg tgtatacaga atagcagaat gggcagacat tacgaatgca cacggtgtgg
     5401 tgggcccagg tattgttagc ggtttgaagc aggcggcaga agaagtaaca aaggaaccta
     5461 gaggcctttt gatgttagca gaattgtcat gcaagggctc cctatctact ggagaatata
     5521 ctaagggtac tgttgacatt gcgaagagcg acaaagattt tgttatcggc tttattgctc
     5581 aaagagacat gggtggaaga gatgaaggtt acgattggtt gattatgaca cccggtgtgg
     5641 gtttagatga caagggagac gcattgggtc aacagtatag aaccgtggat gatgtggtct
     5701 ctacaggatc tgacattatt attgttggaa gaggactatt tgcaaaggga agggatgcta
     5761 aggtagaggg tgaacgttac agaaaagcag gctgggaagc atatttgaga agatgcggcc
     5821 agcaaaacta aaaaactgta ttataagtaa atgcatgtat actaaactca caaattagag
     5881 cttcaattta attatatcag ttattacccg ggaatctcgg tcgtaatgat ttttataatg
     5941 acgaaaaaaa aaaaattgga aagaaaaagc ttggcgtaat catggtcata gctgtttcct
     6001 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6061 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6121 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6181 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6241 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6301 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6361 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6421 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6481 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6541 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     6601 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6661 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6721 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6781 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     6841 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6901 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6961 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7021 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7081 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7141 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7201 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7261 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7321 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7381 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7441 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7501 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7561 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7621 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7681 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7741 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7801 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7861 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7921 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7981 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8041 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8101 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     8161 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
