LOCUS       sequence-79123-e         555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flexed oChIEF-citrine construct in an AAV transfer vector containing
            AAV2 ITR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Lin JY, Lin MZ, Steinbach P, Tsien RY
  TITLE     Characterization of engineered channelrhodopsin variants with 
            improved properties and kinetics.
  JOURNAL   Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034.
  PUBMED    19254539
REFERENCE   2  (bases 1 to 555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2008.11"; journalName: "Biophys J"; date: "2009-03-4-
            4"; volume: "96"; issue: "5"; pages: "1803-14"
COMMENT     Sequence Label: AAV-flex-oChIEF-citrine
FEATURES             Location/Qualifiers
     source          1..555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(438..471)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(522..555)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccgaccccga cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat
       61 cagagagggg gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac
      121 cgcggacagt gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag
      181 gcgcgctgac gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg
      241 tccgcgccgc cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg
      301 ggcgcgacca tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca
      361 gcggaggagt cgtgtcgtgc ctgagagcgc agtcgaattc aagctgatag caaggatcct
      421 ctagagtcga ctccggaata acttcgtata ggatacttta tacgaagtta tgcagaatgg
      481 tagctggatt gtagctgcta ttagcaatat gaaacctctt aataacttcg tatagcatac
      541 attatacgaa gttat
//
