LOCUS       sequence-79129-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains GFP21, which has a sequence similar to YFP, 
            but emission/excitation similar to GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Yamada KM
  TITLE     Yamada Lab Plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pGFP FAK Y397F
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        233..251
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     233..250
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     264..283
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      329..338
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(335..337,338..340,341..853)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(380..401)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(641..660)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
       61 gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc
      121 cccgtgccaa gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt
      181 cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgatttaggt
      241 gacactatag aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca
      301 actgcacctc ggttctatcg attcagccgc caccatggtg agcaagggcg aggagctgtt
      361 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      421 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      481 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg cctacggcct
      541 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      601 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      661 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      721 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      781 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      841 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acaccccc
//
