LOCUS       sequence-79131-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains GFP21, which has a sequence similar to YFP, 
            but emission/excitation similar to GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Tamura M, Gu J, Matsumoto K, Aota S, Parsons R, Yamada KM
  TITLE     Inhibition of cell migration, spreading, and focal adhesions by 
            tumor suppressor PTEN.
  JOURNAL   Science. 1998 Jun 5;280(5369):1614-7.
  PUBMED    9616126
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-06-5"; volume: "280(5369)"; pages: "1614-7"
COMMENT     Sequence Label: pGFP-PTEN D92A
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        229..247
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     229..246
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     260..279
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      325..334
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(331..333,334..336,337..849)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(376..397)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(637..656)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
       61 acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgc ggattccccg
      121 tgccaagagt gacgtaagta ccgcctatag agtctatagg cccaccccct tggcttcgtt
      181 agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca
      241 ctatagaata acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg
      301 cacctcggtt ctatcgattc agccgccacc atggtgagca agggcgagga gctgttcacc
      361 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      421 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      481 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcgccta cggcctgcag
      541 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      601 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      661 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      721 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
      781 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
      841 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaa
//
