LOCUS       sequence-79135-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Koivisto L, Hakkinen L, Matsumoto K, McCulloch CA, Yamada KM, 
            Larjava H
  TITLE     Glycogen synthase kinase-3 regulates cytoskeleton and translocation 
            of Rac1 in long cellular extensions of human keratinocytes.
  JOURNAL   Exp Cell Res. 2004 Feb 1;293(1):68-80.
  PUBMED    14729058
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp Cell 
            Res."; date: "2004-02-1"; volume: "293(1)"; pages: "68-80"
COMMENT     Sequence Label: pRKVSV Rac Q61L
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        226..244
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     226..243
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     257..276
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      322..331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             334..366
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             367..399
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     regulatory      421..430
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgaaccgtc agaatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
       61 ccgggaccga tccagcctcc gcggccggga acggtgcatt ggaacgcgga ttccccgtgc
      121 caagagtgac gtaagtaccg cctatagagt ctataggccc acccccttgg cttcgttaga
      181 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
      241 tagaataaca tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcac
      301 ctcggttcta tcgattcagc cgccaccatg ggatacaccg acatcgagat gaaccggctg
      361 ggcaagtaca ccgacatcga gatgaaccgg ctgggcaagg gatccaagct tctcgaggcc
      421 gccaccatgg gatccaccga cttctaccta aagagatccg ccaccatgca ggccatcaag
      481 tgtgtggtgg tgggagacgg agctgtaggt aaaacttgcc tactgatcag ttacacaacc
      541 aatgcatttc ctggagaata tatccctact gtctttgaca attattctgc caatgttatg
      601 gtagatggaa aaccggtgaa tctgggctta tgggatacag ctggactcga ggattatgac
      661 agattacgcc ccctatccta tccgcaaaca gatgtgttct taatttgctt ttcccttgtg
      721 agtcctgcat catttgaaaa tgtccgtgca aagtggtatc ctgaggtgcg gcaccactgt
      781 cccaacactc ccatcatcct agtgggaact aaacttgatc ttagggatga taaagacacg
      841 atcgagaaac tgaaggagaa gaagctgact c
//
