LOCUS       sequence-79144-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  adeno-associated virus (AAV) encoding a green fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Kaspar BK, Vissel B, Bengoechea T, Crone S, Randolph-Moore L, Muller
            R, Brandon EP, Schaffer D, Verma IM, Lee KF, Heinemann SF, Gage FH
  TITLE     Adeno-associated virus effectively mediates conditional gene 
            modification in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2320-5. Epub 2002 Feb 
            12.
  PUBMED    11842206
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-02-19"; volume: "99(4)"; pages: 
            "2320-5. Epub 2002 Feb 12"
COMMENT     Sequence Label: AAV GFP
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          142..617
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      680..689
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(731..752)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgggac cgatccagcc tcccctcgaa gcttacatgt ggtaccgagc
      121 tcggatcctg agaacttcag ggtgagtcta tgggaccctt gatgttttct ttccccttct
      181 tttctatggt taagttcatg tcataggaag gggagaagta acagggtaca catattgacc
      241 aaatcagggt aattttgcat ttgtaatttt aaaaaatgct ttcttctttt aatatacttt
      301 tttgtttatc ttatttctaa tactttccct aatctctttc tttcagggca ataatgatac
      361 aatgtatcat gcctctttgc accattctaa agaataacag tgataatttc tgggttaagg
      421 caatagcaat atttctgcat ataaatattt ctgcatataa attgtaactg atgtaagagg
      481 tttcatattg ctaatagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg
      541 gataaggctg gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc
      601 ttatcttcct cccacagctc ctgggcaacg tgctggtctg tgtgctggcc catcactttg
      661 gcaaagcacg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
      721 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      781 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      841 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
      901 cagccgctac cccgaccaca tgaagcagca cgacttcttc
//
