LOCUS       sequence-79150-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX330 containing sgRNA against mouse Cent1. Positive control for DSB
            mediated EGFP reconstitution. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M
  TITLE     Generation of mutant mice by pronuclear injection of circular 
            plasmid expressing Cas9 and single guided RNA.
  JOURNAL   Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355.
  PUBMED    24284873
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-27"; volume: "3:3355. doi"; pages: " 
            10.1038/srep03355"
COMMENT     Sequence Label: pX330-Cetn1/1
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccttatcca gttcgctcag gccgcctctc tcggccttgg tcagattgtc gaactttctc
       61 tgggtaatca gcttggcgtt cagcagctgc cgccagtagt tcttcatctt cttcacgacc
      121 tcttcggagg gcacgttgtc gctcttgccc cggttcttgt cgcttctggt cagcaccttg
      181 ttgtcgatgg agtcgtcctt cagaaagctc tgaggcacga tatggtccac atcgtagtcg
      241 gacagccggt tgatgtccag ttcctggtcc acgtacatat cccgcccatt ctgcaggtag
      301 tacaggtaca gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc tttcaggatc
      361 tggctgccca gctctttgat gccctcttcg atccgcttca ttctctcgcg gctgttcttc
      421 tgtcccttct gggtggtctg gttctctctg gccatttcga tcacgatgtt ctcgggcttg
      481 tgccggccca tcactttcac gagctcgtcc accaccttca ctgtctgcag gatgcccttc
      541 ttaatggcgg ggctgccggc cagattggca atgtgctcgt gcaggctatc gccctggccg
      601 gacacctggg ctttctggat gtcctcttta aaggtcaggc tgtcgtcgtg gatcagctgc
      661 atgaagtttc tgttggcgaa gccgtcggac ttcaggaaat ccaggattgt cttgccggac
      721 tgcttgtccc ggatgccgtt gatcagcttc cggctcagcc tgccccagcc ggtgtatctc
      781 cgccgcttca gctgcttcat cactttgtcg tcgaacaggt gggcataggt tttcagccgt
      841 tcctcgatca tctctctgtc ctcaaacagt gtcagggtca gcacgatatc ttccagaatg
      901 tcctcgtttt cctcattgtc caggaagtcc ttgtccttga taattttcag cagatcgtgg
      961 tatgtgccca gggaggcgtt gaaccgatct tccacgcc
//
