LOCUS       sequence-79155-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains the Gateway selection cassette (ccdB ChlorR) flanked by 
            AttR1 and AttR3 sites for the creation of Destination vectors .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R1-ChlorR-R3   #BGV148
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(126..131,132..149,150..156)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(276..295)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
       61 taatactgta aaacacaaca tatccagtca ctatggccgg cgcggccgca ttaggcaccc
      121 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
      181 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
      241 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
      301 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
      361 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
      421 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
      481 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
      541 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
      601 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
      661 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
      721 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
      781 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
      841 actgcgatga gtggcag
//
