LOCUS       sequence-79160-e         327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for recombinant expression in bacteria of SpyLigase. The 
            Howarth lab has created two improved peptide ligation enzymes. See 
            depositor comments below!.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Fierer JO, Veggiani G, Howarth M
  TITLE     SpyLigase peptide-peptide ligation polymerizes affibodies to enhance
            magnetic cancer cell capture.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Mar 17.
  PUBMED    24639550
REFERENCE   2  (bases 1 to 327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Mar 17."
COMMENT     Sequence Label: SpyLigase
FEATURES             Location/Qualifiers
     source          1..327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgacg gtcagtccgg tgacggcaaa
       61 gagttagctg gtgcaactat ggagttgcgt gattcatctg gtaaaactat tagtacatgg
      121 atttcagatg gacaagtgaa agatttctac ctgtatccag gaaaatatac atttgtcgaa
      181 accgcagcac cagacggtta tgaggtagca actgctatta cctttacagt taatgagcaa
      241 ggtcaggtta ctgtaaatgg caaagcaact aaaggtggga gtggtggcag cggaggtagt
      301 ggcgaggaca gcgctaccca tatttaa
//
