LOCUS       sequence-79164-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter and 
            C-terminal Avi-tag, 3C protease cleavage site, GFP and 10x His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXCA3GH
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(56..85)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(101..130)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(137..847)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(177..197)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     792..820
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(854..877)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 agctggagac cgtttaaact caatgatgat gatgatgatg gtcgacggcg ctattcagat
       61 cctcttctga gatgagtttt tgttctagat ggtcgactta atgatggtga tgatgatggt
      121 gatgatgatg actagttttg tagagctcat ccatgccatg tgtaatccca gcagcagtta
      181 caaactcaag aaggaccatg tggtcacgct tttcgttggg atctttcgaa agggcagatt
      241 gtgtcgacag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt
      301 gttgataatg gtctgctagt tgaacggaac catcttcaat gttgtggcga attttgaagt
      361 tagctttgat tccattcttt tgtttgtctg ccgtgatgta tacattgtgt gagttatagt
      421 tgtactctag tttgtgtccg agaatgtttc catcttcttt aaaatcaata ccctttaact
      481 cgatacgatt aacaagggta tcaccttcaa acttgacttc agcacgcgtc ttgtagttcc
      541 cgtcatcttt gaaagatata gtgcgttcct gtacataacc ttcgggcatg gcactcttga
      601 aaaagtcatg ccgtttcatg tgatccggat aacgggaaaa gcattgaaca ccataggtca
      661 gagtagtgac aagtgttggc caaggaacag gtagttttcc agtagtgcaa ataaatttaa
      721 gggtgagttt tccgtatgta gcatcacctt caccctctcc actgacagaa aatttgtgcc
      781 cattaacatc accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc
      841 ctttgctgaa ttgtggacct tgaaacaaaa cttctaattc gtgccattcg attttctgag
      901 cttcgaaga
//
