LOCUS       sequence-79171-e        5290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid contains the coding sequence of MOV10 D645N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Gregersen LH, Schueler M, Munschauer M, Mastrobuoni G, Chen W, Kempa
            S, Dieterich C, Landthaler M
  TITLE     MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target 
            degradation by translocation along 3' UTRs.
  JOURNAL   Mol Cell. 2014 May 22;54(4):573-85. doi: 
            10.1016/j.molcel.2014.03.017. Epub 2014 Apr 10.
  PUBMED    24726324
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.017"; journalName: "Mol Cell"; date: 
            "2014-05-22- 22"; volume: "54"; issue: "4"; pages: "573-85"
COMMENT     Sequence Label: pENTR4 MOV10 D645N
FEATURES             Location/Qualifiers
     source          1..5290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    3106..3117
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(3519..3618)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3730..3749)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3741..4550
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3808..3827)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4640..5228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5129..5148
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc
      481 gacatgccca gtaagttcag ctgccggcag ctccgggagg cgggccagtg tttcgagagt
      541 ttcctggtcg ttcggggact ggacatggag acagatcgcg agcggctgcg gaccatttat
      601 aaccgcgact tcaagatcag ctttgggacc cccgcccctg gcttctcctc catgctgtat
      661 ggaatgaaga ttgcaaatct ggcctacgtc accaagactc gggtcaggtt cttcagactc
      721 gaccgctggg ccgacgtgcg gttcccagaa aagaggagaa tgaagctggg gtcagatatc
      781 agcaaacacc acaagtcact gctagccaag atcttttatg acagggctga gtatcttcat
      841 gggaaacatg gtgtggatgt ggaagtccag gggccccatg aagcccgaga tgggcagctc
      901 cttatccgcc tggatttgaa ccgcaaagag gtgctgaccc tgaggcttcg gaatggcgga
      961 acccagtctg ttaccctcac tcacctcttc ccactctgcc ggacacccca gtttgctttc
     1021 tacaatgaag accaggagtt gccctgtcca ctgggccccg gtgaatgcta tgaactccat
     1081 gtccattgta agaccagctt tgtgggctac ttcccagcca cagtgctctg ggagctgctg
     1141 ggacctgggg agtcgggttc agaaggagcc ggcacattct acattgcccg cttcttggct
     1201 gccgtcgccc acagccccct ggctgcacag ctgaagccca tgactccctt caagcggacc
     1261 cggatcaccg gaaaccctgt ggtgaccaat cggatagagg aaggagagag acctgaccgc
     1321 gctaagggct atgacctgga gttaagtatg gcgctgggga catactaccc acctccccgc
     1381 ctcaggcagc tgctccccat gcttcttcag ggaacaagta tcttcactgc ccctaaggag
     1441 atcgcagaga tcaaggccca gctggagaca gccctgaagt ggaggaacta tgaggtgaag
     1501 ctgcggctgc tgctgcacct ggaggaactg cagatggagc atgatatccg gcactatgac
     1561 ctggagtcgg tgcccatgac ctgggaccct gtggaccaga accccaggct gctcacgctg
     1621 gaggttcctg gagtgactga gagccgcccc tcagtgctac ggggcgacca cctgtttgcc
     1681 cttttgtcct cggagacaca ccaggaggac cccatcacat ataagggctt tgtgcacaag
     1741 gtggaattgg accgtgtcaa gctgagcttt tccatgagcc tcctgagccg ctttgtggat
     1801 gggctgacct tcaaggtgaa ctttaccttc aaccgccagc cgctgcgagt ccagcaccgt
     1861 gccctggagc tgacagggcg ctggctgctg tggcccatgc tctttcctgt ggcacctcgg
     1921 gacgtcccgc tgctgccctc agatgtgaaa ctcaagctgt acgaccggag tctggagtca
     1981 aacccagagc agctgcaggc catgaggcac attgttacgg gcaccacccg tccagccccc
     2041 tacatcatct ttgggcctcc aggcaccggc aagactgtca cgttagtgga ggcaattaag
     2101 caggtggtga agcacttgcc caaagcccac atcttggcct gcgctccatc caactcaggg
     2161 gctgacctac tctgtcaaag gctccgggtc caccttccta gctccatcta ccgcctcctg
     2221 gcccccagca gggacatccg catggtacct gaggacatca agccctgctg caactgggac
     2281 gcaaagaagg gggagtatgt atttcccgcc aagaagaagc tgcaggaata ccgggtctta
     2341 attaccaccc tcatcactgc cggcaggttg gtctcggccc agtttcccat tgatcacttc
     2401 acacacatct tcatcaatga ggctggccac tgcatggagc ctgagagtct ggtagctata
     2461 gcagggctga tggaagtaaa ggaaacaggt gatccaggag ggcagctggt gctggcagga
     2521 gaccctcggc agctggggcc tgtgctgcgt tccccactga cccagaagca tggactggga
     2581 tactcactgc tggagcggct gctcacctac aactccctgt acaagaaggg ccctgatggc
     2641 tatgaccccc agttcataac caagctgctc cgcaactaca ggtctcatcc caccatcctg
     2701 gacattccta accagctcta ttatgaaggg gagctgcagg cctgtgctga tgtcgtggat
     2761 cgagaacgct tctgccgctg ggcgggccta cctcgacagg gctttcccat catctttcac
     2821 ggcgtaatgg gcaaagatga gcgtgaaggc aacagcccat ccttcttcaa ccctgaagag
     2881 gctgccacag tgacttccta cctgaagctg ctcctggccc cctcctccaa gaagggcaaa
     2941 gctcgcctga gccctcgaag tgtgggcgtc atctccccgt accggaaaca ggtggagaaa
     3001 atccgttact gcatcaccaa acttgacagg gagcttcgag gactggatga catcaaggac
     3061 ttgaaggtgg gttcagtaga agaattccaa ggccaagaac gaagcgtcat cctcatctcc
     3121 accgtgcgaa gcagccagag ctttgtgcag ctggatctgg actttaatct gggtttcctt
     3181 aagaacccca agaggttcaa tgtagctgtg acccgggcca aggccctgct catcatcgtg
     3241 gggaaccccc ttctcctggg ccatgaccct gactggaaag tattcctgga gttctgtaaa
     3301 gaaaacggag ggtataccgg gtgtcccttc cctgccaaac tggacctgca acagggacag
     3361 aatttactgc aaggtctgag caagctcagc ccctctacct cagggcccca cagccatgac
     3421 tacctccccc aggagcggga gggtgaaggg ggcctgtctc tgcaagtgga gccagagtgg
     3481 aggaatgagc tctgagcggc cgcactcgag atatctagac ccagctttct tgtacaaagt
     3541 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     3601 aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa aatctctgat
     3661 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     3721 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     3781 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     3841 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     3901 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     3961 cggaatttat gcctcttccg accatcaagc attttatccg tactcctggt gatgcatggt
     4021 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     4081 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     4141 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     4201 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     4261 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     4321 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     4381 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     4441 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     4501 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     4561 ttaattggtt gtaacattat tcagattggg ccccgttcca ctgagcgtca gaccccgtag
     4621 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4681 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4741 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4801 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4861 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4921 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4981 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5041 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5101 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5161 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5221 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5281 ctcacatgtt
//
