LOCUS       sequence-79178-e        8894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid expresses myc-tagged MOV10 K530A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Gregersen LH, Schueler M, Munschauer M, Mastrobuoni G, Chen W, Kempa
            S, Dieterich C, Landthaler M
  TITLE     MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target 
            degradation by translocation along 3' UTRs.
  JOURNAL   Mol Cell. 2014 May 22;54(4):573-85. doi: 
            10.1016/j.molcel.2014.03.017. Epub 2014 Apr 10.
  PUBMED    24726324
REFERENCE   2  (bases 1 to 8894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.017"; journalName: "Mol Cell"; date: 
            "2014-05-22- 22"; volume: "54"; issue: "4"; pages: "573-85"
COMMENT     Sequence Label: pDEST myc MOV10 K530A
FEATURES             Location/Qualifiers
     source          1..8894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(47..65)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(76..93)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(76..92)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(85..107)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    497..631
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(534..553)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     588..607
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      757..1212
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(844..863)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1054..1075
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1331..1688
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1331..1522
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1354..1374)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1539..1674
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1739..2533
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1793..1812)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2403..2422
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2597..2645
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(2686..2705)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2805..2827
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2865..2883)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2951..3055
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3056..3124,3125..3916)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3274..3293)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4087..4675
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4576..4595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4829..4846
                     /label=L4440
                     /note="L4440 vector, forward primer"
     enhancer        5135..5438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5439..5642
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5593..5613
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5639..5663
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5759..5788
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    5798..5822
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    8471..8482
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(join(8884..8894,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggttgatcg cgtgcatgcg acgtcatagc tctctcccta tagtgagtcg
       61 tattataagc taggcactgg ccgtcgtttt acaacgtcgt gactgggaaa actgctagct
      121 tgggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
      181 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactagc
      241 tgcatatgct tgctgcttga gagttttgct tactgagtat gatttatgaa aatattatac
      301 acaggagcta gtgattctaa ttgtttgtgt attttagatt cacagtccca aggctcattt
      361 caggcccctc agtcctcaca gtctgttcat gatcataatc agccatacca catttgtaga
      421 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
      481 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
      541 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
      601 actcatcaat gtatcttatc atgtctggat cgatcctgca ttaatgaatc ggccaacgcg
      661 cggggagagg cggtttgcgt attggctggc gtaatagcga agaggcccgc accgatcgcc
      721 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
      781 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      841 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      901 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      961 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     1021 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     1081 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     1141 attggttaaa aaatgagctg atttaacaaa tatttaacgc gaattttaac aaaatattaa
     1201 cgtttacaat ttcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     1261 cgcatacgcg gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     1321 taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     1381 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     1441 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     1501 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1561 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1621 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1681 tttgcaaaaa gcttgattct tctgacacaa cagtctcgaa cttaaggcta gagccaccat
     1741 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     1801 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     1861 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     1921 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     1981 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     2041 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     2101 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     2161 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     2221 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     2281 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     2341 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     2401 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     2461 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     2521 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     2581 ccatcacgat ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     2641 gtgtgaatcg atagcgataa ggatccgcgt atggtgcact ctcagtacaa tctgctctga
     2701 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2761 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2821 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     2881 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     2941 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3001 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     3061 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3121 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3181 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3241 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     3301 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3361 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     3421 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     3481 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3541 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     3601 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     3661 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     3721 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3781 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3841 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3901 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3961 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4021 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4081 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4141 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4201 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     4261 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4321 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4381 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4441 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     4501 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4561 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4621 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4681 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4741 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4801 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     4861 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gagcttgcaa
     4921 ttcgcgcgtt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4981 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5041 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5101 tagtacgagg ccctttcact cattagatgc atgtcgttac ataacttacg gtaaatggcc
     5161 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     5221 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     5281 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     5341 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     5401 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     5461 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     5521 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     5581 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     5641 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     5701 gaagacaccg ggaccgatcc agcctccgga ctctagccta ggccgcggta ccaccatgga
     5761 acaaaaactt atttctgaag aagatctgtc tagatcaaca agtttgtaca aaaaagcagg
     5821 ctccaccatg ggaaccaatt cagtcgacat gcccagtaag ttcagctgcc ggcagctccg
     5881 ggaggcgggc cagtgtttcg agagtttcct ggtcgttcgg ggactggaca tggagacaga
     5941 tcgcgagcgg ctgcggacca tttataaccg cgacttcaag atcagctttg ggacccccgc
     6001 ccctggcttc tcctccatgc tgtatggaat gaagattgca aatctggcct acgtcaccaa
     6061 gactcgggtc aggttcttca gactcgaccg ctgggccgac gtgcggttcc cagaaaagag
     6121 gagaatgaag ctggggtcag atatcagcaa acaccacaag tcactgctag ccaagatctt
     6181 ttatgacagg gctgagtatc ttcatgggaa acatggtgtg gatgtggaag tccaggggcc
     6241 ccatgaagcc cgagatgggc agctccttat ccgcctggat ttgaaccgca aagaggtgct
     6301 gaccctgagg cttcggaatg gcggaaccca gtctgttacc ctcactcacc tcttcccact
     6361 ctgccggaca ccccagtttg ctttctacaa tgaagaccag gagttgccct gtccactggg
     6421 ccccggtgaa tgctatgaac tccatgtcca ttgtaagacc agctttgtgg gctacttccc
     6481 agccacagtg ctctgggagc tgctgggacc tggggagtcg ggttcagaag gagccggcac
     6541 attctacatt gcccgcttct tggctgccgt cgcccacagc cccctggctg cacagctgaa
     6601 gcccatgact cccttcaagc ggacccggat caccggaaac cctgtggtga ccaatcggat
     6661 agaggaagga gagagacctg accgcgctaa gggctatgac ctggagttaa gtatggcgct
     6721 ggggacatac tacccacctc cccgcctcag gcagctgctc cccatgcttc ttcagggaac
     6781 aagtatcttc actgccccta aggagatcgc agagatcaag gcccagctgg agacagccct
     6841 gaagtggagg aactatgagg tgaagctgcg gctgctgctg cacctggagg aactgcagat
     6901 ggagcatgat atccggcact atgacctgga gtcggtgccc atgacctggg accctgtgga
     6961 ccagaacccc aggctgctca cgctggaggt tcctggagtg actgagagcc gcccctcagt
     7021 gctacggggc gaccacctgt ttgccctttt gtcctcggag acacaccagg aggaccccat
     7081 cacatataag ggctttgtgc acaaggtgga attggaccgt gtcaagctga gcttttccat
     7141 gagcctcctg agccgctttg tggatgggct gaccttcaag gtgaacttta ccttcaaccg
     7201 ccagccgctg cgagtccagc accgtgccct ggagctgaca gggcgctggc tgctgtggcc
     7261 catgctcttt cctgtggcac ctcgggacgt cccgctgctg ccctcagatg tgaaactcaa
     7321 gctgtacgac cggagtctgg agtcaaaccc agagcagctg caggccatga ggcacattgt
     7381 tacgggcacc acccgtccag ccccctacat catctttggg cctccaggca ccggcgcgac
     7441 tgtcacgtta gtggaggcaa ttaagcaggt ggtgaagcac ttgcccaaag cccacatctt
     7501 ggcctgcgct ccatccaact caggggctga cctactctgt caaaggctcc gggtccacct
     7561 tcctagctcc atctaccgcc tcctggcccc cagcagggac atccgcatgg tacctgagga
     7621 catcaagccc tgctgcaact gggacgcaaa gaagggggag tatgtatttc ccgccaagaa
     7681 gaagctgcag gaataccggg tcttaattac caccctcatc actgccggca ggttggtctc
     7741 ggcccagttt cccattgatc acttcacaca catcttcatc gatgaggctg gccactgcat
     7801 ggagcctgag agtctggtag ctatagcagg gctgatggaa gtaaaggaaa caggtgatcc
     7861 aggagggcag ctggtgctgg caggagaccc tcggcagctg gggcctgtgc tgcgttcccc
     7921 actgacccag aagcatggac tgggatactc actgctggag cggctgctca cctacaactc
     7981 cctgtacaag aagggccctg atggctatga cccccagttc ataaccaagc tgctccgcaa
     8041 ctacaggtct catcccacca tcctggacat tcctaaccag ctctattatg aaggggagct
     8101 gcaggcctgt gctgatgtcg tggatcgaga acgcttctgc cgctgggcgg gcctacctcg
     8161 acagggcttt cccatcatct ttcacggcgt aatgggcaaa gatgagcgtg aaggcaacag
     8221 cccatccttc ttcaaccctg aagaggctgc cacagtgact tcctacctga agctgctcct
     8281 ggccccctcc tccaagaagg gcaaagctcg cctgagccct cgaagtgtgg gcgtcatctc
     8341 cccgtaccgg aaacaggtgg agaaaatccg ttactgcatc accaaacttg acagggagct
     8401 tcgaggactg gatgacatca aggacttgaa ggtgggttca gtagaagaat tccaaggcca
     8461 agaacgaagc gtcatcctca tctccaccgt gcgaagcagc cagagctttg tgcagctgga
     8521 tctggacttt aatctgggtt tccttaagaa ccccaagagg ttcaatgtag ctgtgacccg
     8581 ggccaaggcc ctgctcatca tcgtggggaa cccccttctc ctgggccatg accctgactg
     8641 gaaagtattc ctggagttct gtaaagaaaa cggagggtat accgggtgtc ccttccctgc
     8701 caaactggac ctgcaacagg gacagaattt actgcaaggt ctgagcaagc tcagcccctc
     8761 tacctcaggg ccccacagcc atgactacct cccccaggag cgggagggtg aagggggcct
     8821 gtctctgcaa gtggagccag agtggaggaa tgagctctga gcggccgcac tcgagatatc
     8881 tagacccagc tttc
//
