LOCUS       sequence-79183-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid compatible with the Golden Gate TALEN cloning system. To 
            clone TAL effectors 4.5 RVDs long. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, 
            Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
  TITLE     megaTALs: a rare-cleaving nuclease architecture for therapeutic 
            genome engineering.
  JOURNAL   Nucleic Acids Res. 2013 Nov 26.
  PUBMED    24285304
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Nov 26."
COMMENT     Sequence Label: pFUSC4
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    182..203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(218..223,224..241,242..248)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    256..272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     261..283
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     280..296
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     280..296
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             292..669
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLDSH
                     HLVRGQVQGETLKTGRRFTTS"
     primer_bind     315..335
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        317..335
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(382..401)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(383..401)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(411..427)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(643..659)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      723..809
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tccgtttcca cggtgtgcgt cacccggcaa ccttgggtag cagcgaagtc gaggcatttc
       61 tgtcctggct ggtctagacg tctcgatagc caccacttgg tccggggtca ggccatggtc
      121 ctggcacagc accggcaaca ggagaccacc ggtggaaagc gggcagtgag cgcaacgcaa
      181 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      241 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      301 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag gacctcgagg
      361 gggggcccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
      421 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      481 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      541 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgccgg tctcgatagc
      601 caccacttgg tccggggtca ggttcagggg gagacgctta agactggccg tcgttttaca
      661 acgtcgtgac tgggaaaaca tccatgctag cgttaacgcg agagtaggga actgccaggc
      721 atcaaataaa acgaaaggct cagtcggaag actgggcctt tcgttttatc tgttgtttgt
      781 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgtgaagc
      841 aacggcccgg agggtggcgg g
//
