LOCUS       sequence-79184-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains first TALE repeat harboring NG as RVD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: p1NG
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(104..120)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(104..120)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(157..177)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(157..175)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(196..212)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(196..212)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(209..231)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    220..236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(244..250,251..268,269..274))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    289..310
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(427..444)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(678..697)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggttgtcgct attgctagta acggcggagg caaacaggcg ctggaaacag ttcagcgcct
       61 cttgccggtc ttgtgtcagg accacggcct gaccggagac cgggatccac tagttctaga
      121 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg
      181 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      241 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      301 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      361 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      421 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      481 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      541 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      601 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      661 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      721 tcctgttccg accctgccgc ttaccgg
//
