LOCUS       sequence-79193-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes fabD, accA, accB, accC, accD in pseudo-operon 
            configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pACM4-ACA
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggccacga tgcgtccggc gtagcctagg atcgagatcg atctcgatcc cgcgaaatta
       61 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
      121 tttaacttta agaaggagat atacatatga cgcaatttgc atttgtgttc cctggacagg
      181 gttctcaaac cgttggaatg ctggctgata tggcggcgag ctatccaatt gtcgaagaaa
      241 cgtttgctga agcttctgcg gcgctgggct acgacctgtg ggcgctgacc cagcaggggc
      301 cagctgaaga actgaataaa acctggcaaa ctcagcctgc gctgttgact gcatctgttg
      361 cgctgtatcg cgtatggcag cagcagggcg gtaaagcacc ggcaatgatg gccggtcaca
      421 gcctggggga atactccgcg ctggtttgcg ctggtgtgat tgatttcgct gatgcggtgc
      481 gtctggttga gatgcgcggc aagttcatgc aagaagccgt accggaaggc acgggcgcta
      541 tggcggcaat catcggtctg gatgatgcgt ctattgcgaa agcgtgtgaa gaagctgcag
      601 aaggtcaggt cgtttctccg gtaaacttta actctccggg acaggtggtt attgccggtc
      661 ataaagaagc ggttgagcgt gctggcgctg cctgtaaagc ggcgggcgca aaacgcgcgc
      721 tgccgttacc agtgagcgta ccgtctcact gtgcgctgat gaaaccagca gccgacaaac
      781 tggcagt
//
