LOCUS       sequence-79194-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains second TALE repeat harboring NI as RVD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: p2NI
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(146..162)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(146..162)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(199..219)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(199..217)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(238..254)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(238..254)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(251..273)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    262..278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(286..292,293..310,311..316))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    331..352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(469..486)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(720..739)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggtcgacgg tatcgataag cttgggtctc ggaccccaga acaggttgtg gccatcgcca
       61 gcaacatagg tggcaagcag gccctcgaaa ccgtccagag actgttaccg gttctctgcc
      121 aggcccacgg cctgacggag accgggatcc actagttcta gagcggccgc caccgcggtg
      181 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
      241 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      301 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      361 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      421 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      481 ctcgctgctc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      541 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      601 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      661 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      721 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      781 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      841 acgctgta
//
