LOCUS       sequence-79199-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Base vector for PCR-based DNA transplant in yeast. DNA segments to 
            be transplanted into the yeast genome are cloned into the MCS. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Horecka J, Chu AM, Davis RW
  TITLE     IpO: plasmids and methods for simplified, PCR-based DNA transplant 
            in yeast.
  JOURNAL   Yeast. 2014 May;31(5):185-93. doi: 10.1002/yea.3006. Epub 2014 Mar 
            20.
  PUBMED    24604451
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3006"; 
            journalName: "Yeast"; date: "2014-05"; volume: "31"; issue: "5"; 
            pages: "185-93"
COMMENT     Sequence Label: IpO
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..34
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     19..35
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(69..85)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(69..85)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(776..796)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(776..794)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(815..831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(815..831)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(828..850)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    839..855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggaccctgaa accacactcg aggtcgacgg tatcgataag cttgatatcg aattcctgca
       61 gcccggggga tccactagtt ctagagcggc cgggtaataa ctgatataat taaattgaag
      121 ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct
      181 ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct
      241 tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag
      301 agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac
      361 ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt
      421 gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag
      481 tatattctcc agtagctagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc
      541 taggttcctt tgttacttct tccgccgcct gcttcaaacc gctaacaata cctgggccca
      601 ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt
      661 actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat
      721 tgtacttggc ggataatgcc tttagcggct taactgtgag ctccagcttt tgttcccttt
      781 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      841 gttatccgct cacaattcca cacaacata
//
