LOCUS       sequence-79201-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains third TALE repeat harboring NG as RVD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: p3NG
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(103..119)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(103..119)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(156..176)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(156..174)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(195..211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(195..211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(208..230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(243..249,250..267,268..273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(426..443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(677..696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggttgtcgc tattgctagt aacggcggag gcaaacaggc gctggaaaca gttcagcgcc
       61 tcttgccggt cttgtgtcag gcccacggcc tgaggagacc gggatccact agttctagag
      121 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
      181 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      241 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      301 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      361 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      421 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      481 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      541 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      601 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      661 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      721 cctgttccga ccctgccgct tacc
//
