LOCUS       sequence-79203-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains third TALE repeat harboring NI as RVD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: p3NI
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(145..161)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(145..161)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(198..218)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(198..216)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(237..253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(237..253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(250..272)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    261..277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(285..291,292..309,310..315))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    330..351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(468..485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(719..738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtcgacggt atcgataagc ttgggtctcg tgaccccaga ccaggttgtg gccatcgcca
       61 gcaacatagg tggcaagcag gccctcgaaa ccgtccagag actgttaccg gttctctgcc
      121 aggcccacgg cctgaggaga ccgggatcca ctagttctag agcggccgcc accgcggtgg
      181 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
      241 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      301 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      361 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      421 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      481 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      541 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      601 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      661 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      721 agataccagg cgtttccccc tggaagctcc c
//
