LOCUS       sequence-79209-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains fourth TALE repeat harboring NG as RVD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: p4NG
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(111..127)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(111..127)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(164..184)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(164..182)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(203..219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(203..219)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(216..238)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    227..243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(251..257,258..275,276..281))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    296..317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(434..451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(685..704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccccgccca ggttgtcgct attgctagta acggcggagg caaacaggcg ctggaaacag
       61 ttcagcgcct cttgccggtc ttgtgtcagg accacggcct gggagaccgg gatccactag
      121 ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
      181 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      241 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      301 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      361 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      421 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      481 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      541 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      601 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      661 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      721 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      781 gaagcgtggc gctttctcat agctcacgct gta
//
