LOCUS       sequence-79214-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Final destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: ptCMV-136/63-VR-HD
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           62..104
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      112..121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             121..186
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             193..213
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     798..820
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     812..829
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     813..829
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggaagct
       61 tcttgttctt tttgcagaag ctcagaataa acgctcaact ttggcctcga ggccaccatg
      121 gactataagg accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac
      181 gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgta
      241 gatttgagaa ctttgggata ttcacagcag cagcaggaaa agatcaagcc caaagtgagg
      301 tcgacagtcg cgcagcatca cgaagcgctg gtgggtcatg ggtttacaca tgcccacatc
      361 gtagccttgt cgcagcaccc tgcagccctt ggcacggtcg ccgtcaagta ccaggacatg
      421 attgcggcgt tgccggaagc cacacatgag gcgatcgtcg gtgtggggaa acagtggagc
      481 ggagcccgag cgcttgaggc cctgttgacg gtcgcgggag agctgagagg gcctcccctt
      541 cagctggaca cgggccagtt gctgaagatc gcgaagcggg gaggagtcac ggcggtcgag
      601 gcggtacacg cgtggcgcaa tgcgctcacg ggagcacccc tcaaggagac gggcgccgct
      661 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
      721 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
      781 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
      841 atacgactca ctata
//
