LOCUS       sequence-79222-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Final destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: ptCMV-153/47-VR-HD
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           76..118
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      126..135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             147..167
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             177..200
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             201..227
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     primer_bind     851..873
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     865..882
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     866..882
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga agcttcttgt tctttttgca gaagctcaga ataaacgctc aactttggcc
      121 tcgaggccac catggcttcc tcccctccaa agaaaaagag aaaggttgcg gccgctgact
      181 acaaggatga cgacgataaa agttggaagg acgcaagtgg ttggtctaga atgcatgcgg
      241 ccccgcgacg gcgtgctgcg caaccctccg acgcttcgcc ggccgcgcag gtggatctac
      301 gcacgctcgg ctacagtcag cagcagcaag agaagatcaa accgaaggtg cgttcgacag
      361 tggcgcagca ccacgaggca ctggtgggcc atgggtttac acacgcgcac atcgttgcgc
      421 tcagccaaca cccggcagcg ttagggaccg tcgctgtcac gtatcagcac ataatcacgg
      481 cgttgccaga ggcgacacac gaagacatcg ttggcgtcgg caaacagtgg tccggcgcac
      541 gcgccctgga ggccttgctc acggatgcgg gggagttgag aggtccgccg ttacagttgg
      601 acacaggcca acttgtgaag attgcaaaac gtggcggcgt gaccgcaatg gaggcagtgc
      661 atgcatcgcg caatgcgctc acgggagcac ccctcaagga gacgggcgcc gctacagggc
      721 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
      781 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      841 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
      901 tcactat
//
