LOCUS       sequence-79225-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bi-directional expression vector for yeast, constitutively active, 
            LEU2 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Miller CA 3rd, Martinat MA, Hyman LE
  TITLE     Assessment of aryl hydrocarbon receptor complex interactions using 
            pBEVY plasmids: expressionvectors with bi-directional promoters for 
            use in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 1998 Aug 1;26(15):3577-83.
  PUBMED    9671822
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1998-08-1"; volume: "26(15)"; pages: "3577-83"
COMMENT     Sequence Label: pBEVY-L
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(190..294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     362..380
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        400..805
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
       61 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      121 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      181 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      241 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      301 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
      361 aaataggcgt atcacgaggc cctttcgtct tcaagaatta actgtgggaa tactcaggta
      421 tcgtaagatg caagagttcg aatctcttag caaccattat ttttttcctc aacataacga
      481 gaacacacag gggcgctatc gcacagaatc aaattcgatg actggaaatt ttttgttaat
      541 ttcagaggtc gcctgacgca tatacctttt tcaactgaaa aattgggaga aaaaggaaag
      601 gtgagagcgc cggaaccggc ttttcatata gaatagagaa gcgttcatga ctaaatgctt
      661 gcatcacaat acttgaagtt gacaatatta tttaaggacc tattgttttt tccaataggt
      721 ggttagcaat cgtcttactt tctaactttt cttacctttt acatttcagc aatatatata
      781 tatatttcaa ggatatacca ttctaatgtc tgcccctaag aagatcgtcg ttttgccagg
      841 tgaccacgtt ggtcaagaaa tcacagccga agccatta
//
