LOCUS       sequence-79227-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Final destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: ptCMV-153/47-VR-NI
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           73..115
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      123..132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             144..164
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             174..197
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             198..224
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggaagc ttcttgttct ttttgcagaa gctcagaata aacgctcaac tttggcctcg
      121 aggccaccat ggcttcctcc cctccaaaga aaaagagaaa ggttgcggcc gctgactaca
      181 aggatgacga cgataaaagt tggaaggacg caagtggttg gtctagaatg catgcggccc
      241 cgcgacggcg tgctgcgcaa ccctccgacg cttcgccggc cgcgcaggtg gatctacgca
      301 cgctcggcta cagtcagcag cagcaagaga agatcaaacc gaaggtgcgt tcgacagtgg
      361 cgcagcacca cgaggcactg gtgggccatg ggtttacaca cgcgcacatc gttgcgctca
      421 gccaacaccc ggcagcgtta gggaccgtcg ctgtcacgta tcagcacata atcacggcgt
      481 tgccagaggc gacacacgaa gacatcgttg gcgtcggcaa acagtggtcc ggcgcacgcg
      541 ccctggaggc cttgctcacg gatgcggggg agttgagagg tccgccgtta cagttggaca
      601 caggccaact tgtgaagatt gcaaaacgtg gcggcgtgac cgcaatggag gcagtgcatg
      661 catcgcgcaa tgcgctcacg ggagcacccc tcaaggagac gggcgccgct acagggcgcg
      721 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      781 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcc
//
