LOCUS       sequence-79230-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system, 
            for cloning of TALENs and expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALEN(Nd154C63) Ef1a
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(205..225)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(229..255)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(262..315)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cgcctctggc aacgccgtga ttatgtgctg atacgtgaca gcgacggtcc ctaacgctgc
       61 cgggtgttgg ctgagcgcaa cgatgtgcgc gtgtgtaaac ccatggccca ccagtgcctc
      121 gtggtgctgc gccactgtcg aacgcacctt cggtttgatc ttctcttgct gctgctgact
      181 gtagccgagc gtgcgtagat ccacgacttt gcgtttcttc ttaggtggcg cataatctgg
      241 gacatcatat ggatacctgc aggggcccgg attctcctcc acgtcaccgc atgttagaag
      301 acttcctctg ccctctgaat taagcttgtg ccccagtttg ctagggaggt cgcagtatct
      361 ggccactgcc acctcgtgct gctcgacgta ggtctcgttg ttggcctcct tgattctttc
      421 cagtctgtag tccacatagt agacgccagg catcttgagg ttcttagcgg gtttcttgga
      481 tctatatgtg gtcttgatgt ttgcgatcag atggctcccg cccacgagct tcagggccat
      541 gtcgtttctg ccttccaggc cgccgtcagc ggggtacagt gtctcggtga aggcctccca
      601 gccgagtgtt ttcttctgca tcacagggcc gttggatgtg aagttcaccc ctctgatctt
      661 gacgttgtag atgaggcagc cgtcctggag gctggtgtcc tgggtagcgg tcagcacgcc
      721 cccgtcttcg tatgtggtga ctctctccca tgtgaagccc tcagggaagg actgcttgaa
      781 gaagtcgggg atgccctggg tgtggttgat gaaggtcttg ctgccgtaga ggaagctagt
      841 agccaggatg tcgaaggcga aggggagagg gccgccctcg accaccttga ttctcatggt
      901 ctgggtgccc tcgtagggct tgccttcgcc ctcggatgtg cacttgaagt gatggttgtc
      961 cacggtgccc tccatgtaca gcttc
//
