LOCUS       sequence-79231-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system, 
            for cloning of TALENs and expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALEN(Nd154C63) Ef1a
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..36
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(51..56,57..74,75..81)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    89..105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     94..116
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     113..129
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     113..129
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             125..532
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     148..168
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        150..168
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(215..234)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(216..234)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(244..261)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(244..260)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(253..275)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(833..853)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
       61 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      121 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
      181 gaggacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg
      241 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      301 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      361 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      421 ccgtctccag gggggcaccc gtcagtgcat tgcgcgatgc atgcactgcc tccattgcgg
      481 tcacgccgcc acgttttgca atcttcacaa gttggcctgt gtccaactgt aacggcggac
      541 ctctcaactc ccccgcatcc gtgagcaagg cctccagggc gcgtgcgccg gaccactgtt
      601 tgccgacgcc aacgatgtct tcgtgtgtcg cctctggcaa cgccgtgatt atgtgctgat
      661 acgtgacagc gacggtccct aacgctgccg ggtgttggct gagcgcaacg atgtgcgcgt
      721 gtgtaaaccc atggcccacc agtgcctcgt ggtgctgcgc cactgtcgaa cgcaccttcg
      781 gtttgatctt ctcttgctgc tgctgactgt agccgagcgt gcgtagatcc acgactttgc
      841 gtttcttctt aggtggcgca taatctggga catcatatgg a
//
