LOCUS       sequence-79240-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system, 
            for cloning of TALENs and expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALEN(Nd154C63) MND
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             689..742
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             749..775
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             779..799
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
       61 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
      121 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
      181 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
      241 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
      301 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
      361 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
      421 gacactgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
      481 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
      541 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
      601 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
      661 tagcaaactg gggcacaagc ttaattcaga gggcagagga agtcttctaa catgcggtga
      721 cgtggaggag aatccgggcc cctgcaggta tccatatgat gtcccagatt atgcgccacc
      781 taagaagaaa cgcaaagtcg tggatctacg cacgctcggc tacagtcagc agcagcaaga
      841 gaagatcaaa ccgaaggtgc gttcgacagt g
//
