LOCUS       sequence-79245-e         585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system, 
            for cloning of TALENs and expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 585)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALEN(Nd154C63) MND
FEATURES             Location/Qualifiers
     source          1..585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(294..314)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(318..344)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(351..404)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cctctcaact cccccgcatc cgtgagcaag gcctccaggg cgcgtgcgcc ggaccactgt
       61 ttgccgacgc caacgatgtc ttcgtgtgtc gcctctggca acgccgtgat tatgtgctga
      121 tacgtgacag cgacggtccc taacgctgcc gggtgttggc tgagcgcaac gatgtgcgcg
      181 tgtgtaaacc catggcccac cagtgcctcg tggtgctgcg ccactgtcga acgcaccttc
      241 ggtttgatct tctcttgctg ctgctgactg tagccgagcg tgcgtagatc cacgactttg
      301 cgtttcttct taggtggcgc ataatctggg acatcatatg gatacctgca ggggcccgga
      361 ttctcctcca cgtcaccgca tgttagaaga cttcctctgc cctctgaatt aagcttgtgc
      421 cccagtttgc tagggaggtc gcagtatctg gccactgcca cctcgtgctg ctcgacgtag
      481 gtctcgttgt tggcctcctt gattctttcc agtctgtagt ccacatagta gacgccaggc
      541 atcttgaggt tcttagcggg tttcttggat ctatatgtgg tcttg
//
