LOCUS       sequence-79253-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 
            mutated to Ala.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..71
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             99..179
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             204..224
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gagctcggat cctctagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa
       61 aaaagcaggc tggcgccgga accaattcag tcgacatgta cccatacgat gttccagatt
      121 acgcttatcc ttatgacgta cctgactatg cataccctta tgatgtacca gactacgctg
      181 gcgcggtacc aagcggagga agcgagaatc tttattttca gggcactgga ggaacgaatt
      241 caaggatgga ggcccaggcc cacagctcta cggcgactga gaggaagaaa gctgaaaatt
      301 ccattgggaa atgtcccacg agaacagatg tcagcgagaa ggccgtggcc tctagtacca
      361 cttccaatga ggatgaaagt cctggacaga tctatcaccg agagagaaga aacgcaatca
      421 ctatgcagcc tcagagtgtg cagggtctca acaaaatcag tgaggagccc tcgacgtcta
      481 gtgatgagag ggcctcgctg atcaagaaag agatccatgg ctctctacca catctggcgg
      541 agccctctct cccttaccgt gggactgtgt ttgccatgga tccccggaat ggctacatgg
      601 agcctcacta ccaccctcct catcttttcc ctgccttcca tcctcctgta ccaattgatg
      661 ccagacatca tgagggccgt taccattatg atccatctcc tattcctcca ttacatgtgc
      721 cttctgcctt atctagtagc ccgacgtatc cagacttgcc cttcattagg atctccccac
      781 accgta
//
