LOCUS       sequence-79257-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 
            mutated to Ala.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli2 P1-6A
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..73
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             101..181
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             206..226
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatcaac aagtttgtac
       61 aaaaaagcag gctggcgccg gaaccaattc agtcgacatg tatccatacg atgttccaga
      121 ttacgcttat ccttatgacg tacctgacta tgcataccct tatgatgtac cagactacgc
      181 tggcgcggta ccaagcggag gaagcgagaa tctttatttt cagggcactg gaggaacgag
      241 tcgacaaatg gagacttctg ccccagcccc tgcactggag aagaaagaag ccaagagtgg
      301 tctcttggag gacagcagct tccccgaccc agggaaaaag gcctgtcctc tggcggtggc
      361 cgcagctgta gccgcccacg gagttcctca gcagctcctg ccggctttcc acgcgccttt
      421 gccgattgac atgagacacc aggagggaag gtaccattat gaccctcact ctgtccacag
      481 tgtacacggg cctcccaccc taagtggcag ccctgtcatc tcagatatct ccttgatacg
      541 actttctcca caccctgctg gccctggaga gtcacccttc agcgcccacc acccctacgt
      601 gaacccccat atggagcact acctccggtc tgtgcacagc agccccacac tctcaatgat
      661 ctctgccgcc aggggcctca gccctgctga tgtggcccac gaacatctga aagagagggg
      721 actctttagc ctcgcagccc caggcaccaa cccttcagac tattaccacc agatgaccct
      781 catggcaagc caccccaccc cttatgggg
//
