LOCUS       sequence-79260-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to
            Ala .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    36..60
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             88..168
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             193..213
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cactagtcca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagcaggct
       61 ggcgccggaa ccaattcagt cgacatgtac ccatacgatg ttccagatta cgcttatcct
      121 tatgacgtac ctgactatgc atacccttat gatgtaccag actacgctgg cgcggtacca
      181 agcggaggaa gcgagaatct ttattttcag ggcactggag gaacgaattc aaggatggag
      241 gcccaggccc acagctctac ggcgactgag aggaagaaag ctgaaaattc cattgggaaa
      301 tgtcccacga gaacagatgt cagcgagaag gccgtggcct ctagtaccac ttccaatgag
      361 gatgaaagtc ctggacagat ctatcaccga gagagaagaa acgcaatcac tatgcagcct
      421 cagagtgtgc agggtctcaa caaaatcagt gaggagccct cgacgtctag tgatgagagg
      481 gcctcgctga tcaagaaaga gatccatggc tctctaccac atctggcgga gccctctctc
      541 ccttaccgtg ggactgtgtt tgccatggat ccccggaatg gctacatgga gcctcactac
      601 caccctcctc atcttttccc tgccttccat cctcctgtac caattgatgc cagacatcat
      661 gagggccgtt accattatga tccatctcct attcctccat tacatgtgcc ttctgcctta
      721 tctagtagcc cgacgtatcc agacttgccc ttcattagga tctccccaca ccgtaatccc
      781 actgcagcct caga
//
