LOCUS       sequence-79261-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system, 
            for cloning of TALENs and expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALEN(Nd154C63) SFFV
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             119..820
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MASELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     CDS             824..877
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             884..910
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
       61 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccgat
      121 ggctagcgag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga
      181 caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
      241 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
      301 tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt
      361 caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
      421 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
      481 gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg
      541 ggaggccttc accgagacac tgtaccccgc tgacggcggc ctggaaggca gaaacgacat
      601 ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc
      661 caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga
      721 aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag
      781 atactgcgac ctccctagca aactggggca caagcttaat tcagagggca gaggaagtct
      841 tctaacatgc ggtgacgtgg aggagaatcc gggcccctgc aggtatccat atgatgtccc
      901 agattatgcg ccacct
//
