LOCUS       sequence-79262-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli expression vector for His8-SUMO tagged recombinant protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  TITLE     pCIOX
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCIOX
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    68..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             107..129
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             149..172
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             182..199
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             206..499
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             611..628
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(681..699)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca
       61 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
      121 agaaggagat atacatatgg gcagcagcca tcaccatcat catcatcatc acggcagcgg
      181 cctggtgccg cgcggcagcg ctagcatgtc ggactcagaa gtcaatcaag aagctaagcc
      241 agaggtcaag ccagaagtca agcctgagac tcacatcaat ttaaaggtgt ccgatggatc
      301 ttcagagatc ttcttcaaga tcaaaaagac cactccttta agaaggctga tggaagcgtt
      361 cgctaaaaga cagggtaagg aaatggactc cttaagattc ttgtacgacg gtattagaat
      421 acaagctgat cagacccctg aagatttgga catggaggat aacgatatta ttgaggctca
      481 cagagaacag attggtggtc gcggatccga attcgagctc cgtcgacaag cttgcggccg
      541 cactcgagag actagcttag gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa
      601 cttagtcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
      661 agctgagttg gctgctgcca ccgctgagca ataactagca taacccc
//
