LOCUS       sequence-79266-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system. 
            For cloning of megaTALs or other TAL-fusions and expression in 
            mammalian cells. Meganuclease/fusion is cloned between Xba-Sal 
            sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, 
            Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
  TITLE     megaTALs: a rare-cleaving nuclease architecture for therapeutic 
            genome engineering.
  JOURNAL   Nucleic Acids Res. 2013 Nov 26.
  PUBMED    24285304
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Nov 26."
COMMENT     Sequence Label: pCVL TAL(Nd154C63) SFFV Xba-Sal linker
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..38
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        20..38
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(85..104)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(86..104)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(114..131)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(114..130)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(123..145)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(703..723)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(727..753)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(760..813)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gaggacctcg
       61 agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
      121 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      181 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      241 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc ccgtctccag
      301 gggggcaccc gtcagtgcat tgcgcgatgc atgcactgcc tccattgcgg tcacgccgcc
      361 acgttttgca atcttcacaa gttggcctgt gtccaactgt aacggcggac ctctcaactc
      421 ccccgcatcc gtgagcaagg cctccagggc gcgtgcgccg gaccactgtt tgccgacgcc
      481 aacgatgtct tcgtgtgtcg cctctggcaa cgccgtgatt atgtgctgat acgtgacagc
      541 gacggtccct aacgctgccg ggtgttggct gagcgcaacg atgtgcgcgt gtgtaaaccc
      601 atggcccacc agtgcctcgt ggtgctgcgc cactgtcgaa cgcaccttcg gtttgatctt
      661 ctcttgctgc tgctgactgt agccgagcgt gcgtagatcc acgactttgc gtttcttctt
      721 aggtggcgca taatctggga catcatatgg atacctgcag gggcccggat tctcctccac
      781 gtcaccgcat gttagaagac ttcctctgcc ctctgaatta agcttgtgcc ccagtttgct
      841 agggaggtcg cagtatctgg ccactgccac ctcgtgctgc tcgacgtagg tctcgttgtt
      901 ggcctccttg attctttcca gtctgtagtc cacatagtag acgccaggca tcttgaggtt
      961 cttagcgggt ttcttggatc tatatgtggt cttgat
//
