LOCUS       sequence-79267-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system. 
            For cloning of megaTALs or other TAL-fusions and expression in 
            mammalian cells. Meganuclease/fusion is cloned between Xba-Sal 
            sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, 
            Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
  TITLE     megaTALs: a rare-cleaving nuclease architecture for therapeutic 
            genome engineering.
  JOURNAL   Nucleic Acids Res. 2013 Nov 26.
  PUBMED    24285304
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Nov 26."
COMMENT     Sequence Label: pCVL TAL(Nd154C63) SFFV Xba-Sal linker
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(73..93)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(73..91)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(112..128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..128)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(125..147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    136..152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(160..166,167..184,185..190))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    205..226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcctc
       61 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
      121 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      181 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      241 actgcccgct ttccaccggt cgtctccaac gaccacctcg tcgccttggc ctgcctcggc
      301 ggacgtcctg ccatggatgc agtgaaaaag ggattgccgc acgcgccgga attgatcaga
      361 agagtcaatc gccgtattgg cgaacgcacg tcccatcgcg ttgcgatatc tagatgacgt
      421 agtgatctga tctcagtcga ccgagcatct taccgccatt tatacccata tttgttctgt
      481 ttttcttgat ttgggtatac atttaaatgt taatagaaca aaatggtggg gcaatcattt
      541 acatttttag ggatatgtaa ttactagttc aggtgtattg ccacaagaca aacatgttaa
      601 gaaactttcc cgttatttac gctctgttcc tgttaatcaa cctctggatt acaaaatttg
      661 tgaaagattg actgatattc ttaactatgt tgctcctttt acgctgtgtg gatatgctgc
      721 tttatagcct ctgtatctag ctattgcttc ccgtacggct ttcgttttct cctccttgta
      781 taaatcctgg ttgctgtctc ttttagagga gttgtggccc gttgtccgtc aacgtggcgt
      841 ggtgtgctct gtgtttgctg acgcaacccc cactggctgg ggcattgcca ccacctgtca
      901 actcctttct gggactttcg ctttccc
//
