LOCUS       sequence-79271-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Final destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: ptCMV-136/63-VR-NN
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(61..81)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(61..79)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(100..116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(100..116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(113..135)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    124..140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(148..154,155..172,173..178))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    193..214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcctcca gcttttgttc
       61 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
      121 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      181 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      241 ccaccggtcg tctccacccc tgagcaggta gtggctattg catccaacaa cgggggcaga
      301 cccgcactgg agtcaatcgt ggcccagctt tcgaggccgg accccgcgct ggccgcactc
      361 actaatgatc atcttgtagc gctggcctgc ctcggcggac gacccgcctt ggatgcggtg
      421 aagaaggggc tcccgcacgc gcctgcattg attaagcgga ccaacagaag gatccccgag
      481 aggacatcac atcgagtggc aggttcccaa ctcgtgaaga gtgaacttga ggagaaaaag
      541 tcggagctgc ggcacaaatt gaaatacgta ccgcatgaat acatcgaact tatcgaaatt
      601 gctaggaact cgactcaaga cagaatcctt gagatgaagg taatggagtt ctttatgaag
      661 gtttatggat accgagggaa gcatctcggt ggatcacgaa aacccgacgg agcaatctat
      721 acggtgggga gcccgattga ttacggagtg atcgtcgaca cgaaagccta cagcggtggg
      781 tacaatcttc ccatcgggca ggcagatgag atgcaacgtt atgtcgaaga aaatcagacc
      841 aggaacaaac acatcaatcc aaatgagtgg tggaaagtgt atccttcatc agtgaccgag
      901 tttaagtttt tgtttgtctc tgggcatttc aa
//
