LOCUS       sequence-79280-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 
            promoter for genome editing. SV40 NLS is fused to the C terminal. 
            GAL4 region from pACT2 is removed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Gao Y, Zhao Y
  TITLE     Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro 
            and in vivo for CRISPR-mediated genome editing.
  JOURNAL   J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
  PUBMED    24373158
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152."
COMMENT     Sequence Label: pACT2-CAS9c
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgctatcaag tataaataga cctgcaatta ttaatctttt gtttcctcgt cattgttctc
       61 gttccctttc ttccttgttt ctttttctgc acaatatttc aagctatacc aagcatacaa
      121 tcaactccaa tggacaagaa gtactccatt gggctcgata tcggcacaaa cagcgtcggc
      181 tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat
      241 accgatcgcc acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggag
      301 acggccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat
      361 cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc
      421 ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca
      481 atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat
      541 ctgaggaaga agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg
      601 ctggcgcata tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac
      661 aacagcgatg tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa
      721 gagaacccga tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc
      781 aaatcccggc ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg
      841 tttggtaatc ttatcgccct gtcactcggg ctgaccccca actttaaatc taacttcgac
      901 ctggccgaag atgccaagct tcaactgagc aaagacacc
//
