LOCUS       sequence-79281-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 
            promoter for genome editing. SV40 NLS is fused to the C terminal. 
            GAL4 region from pACT2 is removed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Gao Y, Zhao Y
  TITLE     Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro 
            and in vivo for CRISPR-mediated genome editing.
  JOURNAL   J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
  PUBMED    24373158
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152."
COMMENT     Sequence Label: pACT2-CAS9c
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(159..179)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atcataaatc ataagaaatt cgcttattta gaagtgtcaa caacgtatct accaacgatt
       61 tgaccctttt ccatcttttc gtaaatttct ggcaaggtag acaagccgac aaccttgatt
      121 ggagacttga ccaaacctct ggcgaagaag tccaatcaca ccttcctctt cttcttgggg
      181 tcagccctgc tgtctccacc gagctgagag aggtcgattc ttgtttcata gagccccgta
      241 attgactgat gaatcagtgt ggcgtccagg acctcctttg tagaggtgta ccgctttctg
      301 tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc ccaagttggt cagagtaaac
      361 aagtggataa tgttttctgc ctgctccctg atgggcttat ccctgtgctt attgtaagca
      421 gaaagcacct tatcgaggtt agcgtcggcg aggatcactc ttttggagaa ttcgcttatt
      481 tgctcgatga tctcatcaag gtagtgtttg tgttgttcca cgaacagctg cttctgctca
      541 ttatcttcgg gagacccttt gagcttttca tagtggctgg ccagatacaa gaaattaacg
      601 tatttagagg gcagtgccag ctcgttacct ttctgcagct cgcccgcact agcgagcatt
      661 cgtttccggc cgttttcaag ctcaaagaga gagtacttgg gaagcttaat gatgaggtct
      721 tttttgacct ctttatatcc tttcgcctcg agaaagtcga tggggttttt ttcgaagctt
      781 gatcgctcca tgattgtgat gcccagcagt tccttgacgc ttttgagttt tttagacttc
      841 cctttctcca ctttggccac aaccagtaca ctgtaagcga ctgtaggaga atcgaatccg
      901 ccgtatttct tggggtccca atcttttttg cgtgcgatca gcttgtcgct gttccttttc
//
