LOCUS       sequence-79282-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as pRS316-RGR-GFP except for the HH ribozyme region which has a
            13 bp deletion at its 5' end..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Gao Y, Zhao Y
  TITLE     Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro 
            and in vivo for CRISPR-mediated genome editing.
  JOURNAL   J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
  PUBMED    24373158
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152."
COMMENT     Sequence Label: pRS316-RGR-GFP-mHH
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        79..480
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     misc_RNA        531..606
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           611..678
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
ORIGIN
        1 accgcggtgg cggccgctct agaactagtg ggagctgcat gtgtcagagg ttttcaccgt
       61 catcaccgaa acgcgcgagg cagcaacttc ttttcttttt ttttcttttc tctctccccc
      121 gttgttgtct caccatatcc gcaatgacaa aaaaatgatg gaagacacta aaggaaaaaa
      181 ttaacgacaa agacagcacc aacagatgtc gttgttccag agctgatgag gggtatctcg
      241 aagcacacga aactttttcc ttccttcatt cacgcacact actctctaat gagcaacggt
      301 atacggcctt ccttccagtt acttgaattt gaaataaaaa aaagtttgct gtcttgctat
      361 caagtataaa tagacctgca attattaatc ttttgtttcc tcgtcattgt tctcgttccc
      421 tttcttcctt gtttcttttt ctgcacaata tttcaagcta taccaagcat acaatcaact
      481 gtccgtgagg acgaaacgag taagctcgtc cgtgctgaag tcaagtttga gttttagagc
      541 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      601 cggtgctttt ggccggcatg gtcccagcct cctcgctggc gccggctggg caacatgctt
      661 cggcatggcg aatgggacag ctttggactt cttcgccaga ggtttggtca agtctccaat
      721 caaggttgtc ggcttgtcta ccttgccaga aatttacgaa aagatggaaa agggtcaaat
      781 cgttggtaga tacgttgttg acacttctaa ataagcgaat ttcttatgat ttatgatttt
      841 tattattaaa taagttataa aaaaa
//
